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Role of altholactone in inducing type II apoptosis signalling pathway and expression of cancer-related genes in cervical carcinoma HeLa cell line

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dc.contributor.authorUthaisang-Tanechpongtamb W.
dc.contributor.authorSriyabhaya P.
dc.contributor.authorWilairat P.
dc.date.accessioned2021-04-05T03:33:05Z
dc.date.available2021-04-05T03:33:05Z
dc.date.issued2013
dc.date.issuedBE2556
dc.description.abstractGoniothalamus species (Annonaceae) is a shrub that grows in the rainforest of tropical Asia. Several compounds have been isolated and exhibit the potential use for cancer treatment. In this work, altholactone isolated from Goniothalamus macrophyllus was investigated for its cytotoxicity, apoptosis signalling and the expression of cancer-related genes in the cervical carcinoma HeLa cells. Cytotoxicity was evaluated by MTT assay. Apoptotic characteristics were evaluated by morphological studies. Caspase-3 activity was detected using a fluorogenic substrate. Cytochrome c release from mitochondria and protein Bid were determined byWestern blotting and cancer-related genes expression by RT-PCR. The results demonstrated that altholactone was cytotoxic to HeLa (IC50 = 9.6 μmg/mL), and apoptotic cell death was manifested by appearance of chromatin condensation and caspase-3 activation, which was inhibited by specific inhibitors of both caspase-8 and -9. Release into the cytosol of cytochrome and cleavage of Bid occurred. Altholactone also caused a decrease in bcl-2 and an increase in p53 expression. These unique properties of altholactone suggest a potential for cancer chemotherapy. © 2013 International Federation for Cell Biology.
dc.format.mimetypeapplication/pdf
dc.identifier.citationCell Biology International. Vol 37, No.5 (2013), p.471-477
dc.identifier.doi10.1002/cbin.10059
dc.identifier.issn10656995
dc.identifier.other2-s2.0-84878137096
dc.identifier.urihttps://hdl.handle.net/20.500.14740/6662
dc.rights.holderมหาวิทยาลัยศรีนครินทรวิโรฒ
dc.subject.otherCaspase 3
dc.subject.otherCaspase 8
dc.subject.otherCaspase 9
dc.subject.otherCytochrome c
dc.subject.otherGoniothalenol
dc.subject.otherProtein bcl 2
dc.subject.otherProtein Bid
dc.subject.otherProtein p53
dc.subject.otherApoptosis
dc.subject.otherArticle
dc.subject.otherBcl 2 gene
dc.subject.otherCancer chemotherapy
dc.subject.otherCarcinoma cell
dc.subject.otherCell structure
dc.subject.otherChromatin condensation
dc.subject.otherControlled study
dc.subject.otherCytotoxicity
dc.subject.otherDrug determination
dc.subject.otherDrug efficacy
dc.subject.otherDrug mechanism
dc.subject.otherDrug structure
dc.subject.otherEnzyme activation
dc.subject.otherEnzyme activity
dc.subject.otherEnzyme inhibition
dc.subject.otherEnzyme release
dc.subject.otherGene activity
dc.subject.otherGene expression profiling
dc.subject.otherGene function
dc.subject.otherGoniothalamus
dc.subject.otherGoniothalamus macrophyllus
dc.subject.otherHuman
dc.subject.otherHuman cell
dc.subject.otherIntracellular signaling
dc.subject.otherMitochondrion
dc.subject.otherOncogene
dc.subject.otherP53 gene
dc.subject.otherProtein analysis
dc.subject.otherProtein cleavage
dc.subject.otherUterine cervix carcinoma
dc.titleRole of altholactone in inducing type II apoptosis signalling pathway and expression of cancer-related genes in cervical carcinoma HeLa cell line
dc.typeArticle
dspace.entity.typePublication
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