Publication:
Role of altholactone in inducing type II apoptosis signalling pathway and expression of cancer-related genes in cervical carcinoma HeLa cell line

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Issued Date

2013

Resource Type

Article

File Type

application/pdf

ISSN

10656995

DOI

10.1002/cbin.10059

Other identifier(s)

2-s2.0-84878137096

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Cell Biology International. Vol 37, No.5 (2013), p.471-477

Suggested Citation

Uthaisang-Tanechpongtamb W., Sriyabhaya P., Wilairat P. Role of altholactone in inducing type II apoptosis signalling pathway and expression of cancer-related genes in cervical carcinoma HeLa cell line. Cell Biology International. Vol 37, No.5 (2013), p.471-477. doi:10.1002/cbin.10059 Retrieved from: https://hdl.handle.net/20.500.14740/6662

Title

Role of altholactone in inducing type II apoptosis signalling pathway and expression of cancer-related genes in cervical carcinoma HeLa cell line

Author(s)

Uthaisang-Tanechpongtamb W.
 Sriyabhaya P.
 Wilairat P.

Abstract

Goniothalamus species (Annonaceae) is a shrub that grows in the rainforest of tropical Asia. Several compounds have been isolated and exhibit the potential use for cancer treatment. In this work, altholactone isolated from Goniothalamus macrophyllus was investigated for its cytotoxicity, apoptosis signalling and the expression of cancer-related genes in the cervical carcinoma HeLa cells. Cytotoxicity was evaluated by MTT assay. Apoptotic characteristics were evaluated by morphological studies. Caspase-3 activity was detected using a fluorogenic substrate. Cytochrome c release from mitochondria and protein Bid were determined byWestern blotting and cancer-related genes expression by RT-PCR. The results demonstrated that altholactone was cytotoxic to HeLa (IC50 = 9.6 μmg/mL), and apoptotic cell death was manifested by appearance of chromatin condensation and caspase-3 activation, which was inhibited by specific inhibitors of both caspase-8 and -9. Release into the cytosol of cytochrome and cleavage of Bid occurred. Altholactone also caused a decrease in bcl-2 and an increase in p53 expression. These unique properties of altholactone suggest a potential for cancer chemotherapy. © 2013 International Federation for Cell Biology.

Subject(s)

Caspase 3
 Caspase 8
 Caspase 9
 Cytochrome c
 Goniothalenol
 Protein bcl 2
 Protein Bid
 Protein p53
 Apoptosis
 Article
 Bcl 2 gene
 Cancer chemotherapy
 Carcinoma cell
 Cell structure
 Chromatin condensation
 Controlled study
 Cytotoxicity
 Drug determination
 Drug efficacy
 Drug mechanism
 Drug structure
 Enzyme activation
 Enzyme activity
 Enzyme inhibition
 Enzyme release
 Gene activity
 Gene expression profiling
 Gene function
 Goniothalamus
 Goniothalamus macrophyllus
 Human
 Human cell
 Intracellular signaling
 Mitochondrion
 Oncogene
 P53 gene
 Protein analysis
 Protein cleavage
 Uterine cervix carcinoma

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URI

https://hdl.handle.net/20.500.14740/6662

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Scopus: Year 1983-2021

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