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Improved resolution and sensitivity of human DNA fingerprinting by specific-primed labelling of M13 DNA

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dc.contributor.authorTassanakajon A.
dc.contributor.authorWongteerasapaya C.
dc.contributor.authorPumichoti P.
dc.contributor.authorBoonsaeng V.
dc.contributor.authorPanyim S.
dc.date.accessioned2021-04-05T04:33:56Z
dc.date.available2021-04-05T04:33:56Z
dc.date.issued1991
dc.date.issuedBE2534
dc.description.abstractA method to label M13 DNA probe by primer extension using a specific oligonucleotide primer is described. The method specifically labels the two 15-bp repeats in M13 DNA which hybridize to target DNA giving rise to DNA fingerprinting patterns. The M13 probe labelled by this method gave superior DNA fingerprinting patterns than that labelled by random primers. As little as 0.25 μg of target DNA was sufficient for DNA fingerprinting. Non-isotopic labelling by the specific primer also showed improved DNA fingerprinting pattern. The results demonstrate the methodology to improve DNA fingerprinting based on M13 DNA probe. © 1991.
dc.format.mimetypeapplication/pdf
dc.identifier.citationMolecular and Cellular Probes. Vol 5, No.2 (1991), p.111-115
dc.identifier.doi10.1016/0890-8508(91)90004-4
dc.identifier.issn8908508
dc.identifier.other2-s2.0-0026149814
dc.identifier.urihttps://hdl.handle.net/20.500.14740/7516
dc.rights.holderScopus
dc.subject.otherDeoxyribonucleases, Type II Site Specific
dc.subject.otherDigoxigenin
dc.subject.otherEndodeoxyribonuclease HaeIII
dc.subject.otherPhosphorus
dc.subject.otherType II site specific deoxyribonuclease
dc.subject.otherArticle
dc.subject.otherDNA fingerprinting
dc.subject.otherDNA probe
dc.subject.otherHuman
dc.subject.otherMethodology
dc.subject.otherMolecular genetics
dc.subject.otherNucleic acid hybridization
dc.subject.otherNucleotide repeat
dc.subject.otherNucleotide sequence
dc.subject.otherBase Sequence
dc.subject.otherDeoxyribonucleases, Type II Site-Specific
dc.subject.otherDigoxigenin
dc.subject.otherDNA Fingerprinting
dc.subject.otherDNA Probes
dc.subject.otherHuman
dc.subject.otherMolecular Sequence Data
dc.subject.otherNucleic Acid Hybridization
dc.subject.otherPhosphorus Radioisotopes
dc.subject.otherRepetitive Sequences, Nucleic Acid
dc.subject.otherSupport, Non-U.S. Gov't
dc.titleImproved resolution and sensitivity of human DNA fingerprinting by specific-primed labelling of M13 DNA
dc.typeArticle
dspace.entity.typePublication
swu.datasource.scopushttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0026149814&doi=10.1016%2f0890-8508%2891%2990004-4&partnerID=40&md5=bea540187a8a3cf0724664178af048e0

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