Publication:
Improved resolution and sensitivity of human DNA fingerprinting by specific-primed labelling of M13 DNA

2
0

Issued Date

1991

Resource Type

Article

File Type

application/pdf

ISSN

8908508

DOI

10.1016/0890-8508(91)90004-4

Other identifier(s)

2-s2.0-0026149814

Rights Holder(s)

Scopus

Bibliographic Citation

Molecular and Cellular Probes. Vol 5, No.2 (1991), p.111-115

Suggested Citation

Tassanakajon A., Wongteerasapaya C., Pumichoti P., Boonsaeng V., Panyim S. Improved resolution and sensitivity of human DNA fingerprinting by specific-primed labelling of M13 DNA. Molecular and Cellular Probes. Vol 5, No.2 (1991), p.111-115. doi:10.1016/0890-8508(91)90004-4 Retrieved from: https://hdl.handle.net/20.500.14740/7516

Title

Improved resolution and sensitivity of human DNA fingerprinting by specific-primed labelling of M13 DNA

Author(s)

Tassanakajon A.
 Wongteerasapaya C.
 Pumichoti P.
 Boonsaeng V.
 Panyim S.

Abstract

A method to label M13 DNA probe by primer extension using a specific oligonucleotide primer is described. The method specifically labels the two 15-bp repeats in M13 DNA which hybridize to target DNA giving rise to DNA fingerprinting patterns. The M13 probe labelled by this method gave superior DNA fingerprinting patterns than that labelled by random primers. As little as 0.25 μg of target DNA was sufficient for DNA fingerprinting. Non-isotopic labelling by the specific primer also showed improved DNA fingerprinting pattern. The results demonstrate the methodology to improve DNA fingerprinting based on M13 DNA probe. © 1991.

Subject(s)

Deoxyribonucleases, Type II Site Specific
 Digoxigenin
 Endodeoxyribonuclease HaeIII
 Phosphorus
 Type II site specific deoxyribonuclease
 Article
 DNA fingerprinting
 DNA probe
 Human
 Methodology
 Molecular genetics
 Nucleic acid hybridization
 Nucleotide repeat
 Nucleotide sequence
 Base Sequence
 Deoxyribonucleases, Type II Site-Specific
 Digoxigenin
 DNA Fingerprinting
 DNA Probes
 Human
 Molecular Sequence Data
 Nucleic Acid Hybridization
 Phosphorus Radioisotopes
 Repetitive Sequences, Nucleic Acid
 Support, Non-U.S. Gov't

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URI

https://hdl.handle.net/20.500.14740/7516

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Scopus: Year 1983-2021

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