Publication:
Purification and characterization of organic solvent and detergent tolerant lipase from thermotolerant Bacillus sp. RN2

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dc.contributor.authorKanjanavas P.
dc.contributor.authorKhuchareontaworn S.
dc.contributor.authorKhawsak P.
dc.contributor.authorPakpitcharoen A.
dc.contributor.authorPothivejkul K.
dc.contributor.authorSantiwatanakul S.
dc.contributor.authorMatsui K.
dc.contributor.authorKajiwara T.
dc.contributor.authorChansiri K.
dc.date.accessioned2021-04-05T03:36:21Z
dc.date.available2021-04-05T03:36:21Z
dc.date.issued2010
dc.date.issuedBE2553
dc.description.abstractThe aim of this study was to characterize the organic solvent and detergent tolerant properties of recombinant lipase isolated from thermotolerant Bacillus sp. RN2 (Lip-SBRN2). The isolation of the lipase-coding gene was achieved by the use of inverse and direct PCR. The complete DNA sequencing of the gene revealed that the lip-SBRN2 gene contains 576 nucleotides which corresponded to 192 deduced amino acids. The purified enzyme was homogeneous with the estimated molecular mass of 19 kDa as determined by SDS-PAGE and gel filtration. The Lip-SBRN2 was stable in a pH range of 9-11 and temperature range of 45-60 °C. The enzyme was a non metallo-monomeric protein and was active against pNP-caprylate (C8) and pNP-laurate (C12) and coconut oil. The Lip-SBRN2 exhibited a high level of activity in the presence of 108% benzene, 102.4% diethylether and 112% SDS. It is anticipated that the organic solvent and detergent tolerant enzyme secreted by Bacillus sp. RN2 will be applicable as catalysts for reaction in the presence of organic solvents and detergents. © 2010 by the authors; licensee MDPI.
dc.format.mimetypeapplication/pdf
dc.identifier.citationInternational Journal of Molecular Sciences. Vol 11, No.10 (2010), p.3783-3792
dc.identifier.doi10.3390/ijms11103783
dc.identifier.issn14220067
dc.identifier.other2-s2.0-77958115682
dc.identifier.urihttps://hdl.handle.net/20.500.14740/7535
dc.rights.holderมหาวิทยาลัยศรีนครินทรวิโรฒ
dc.subject.other4 nitrophenyl caprylate
dc.subject.otherBacterial enzyme
dc.subject.otherBenzene
dc.subject.otherCoconut oil
dc.subject.otherDetergent
dc.subject.otherEdetic acid
dc.subject.otherEsterase inhibitor
dc.subject.otherEther
dc.subject.otherLauric acid 4 nitrophenyl ester
dc.subject.otherLauric acid derivative
dc.subject.otherMetal ion
dc.subject.otherOctanoic acid
dc.subject.otherOctanoic acid derivative
dc.subject.otherOrganic solvent
dc.subject.otherRecombinant enzyme
dc.subject.otherRN2 protein
dc.subject.otherTriacylglycerol lipase
dc.subject.otherUnclassified drug
dc.subject.otherArticle
dc.subject.otherBacillus
dc.subject.otherBacterial strain
dc.subject.otherBiocatalyst
dc.subject.otherControlled study
dc.subject.otherDNA sequence
dc.subject.otherEnzyme activity
dc.subject.otherEnzyme analysis
dc.subject.otherEnzyme isolation
dc.subject.otherEnzyme purification
dc.subject.otherEnzyme specificity
dc.subject.otherEnzyme stability
dc.subject.otherGel filtration
dc.subject.otherGenetic code
dc.subject.otherHeat tolerance
dc.subject.otherMolecular weight
dc.subject.otherNonhuman
dc.subject.otherNucleotide sequence
dc.subject.otherPH measurement
dc.subject.otherPolyacrylamide gel electrophoresis
dc.subject.otherPolymerase chain reaction
dc.subject.otherBacillus
dc.subject.otherDetergent tolerant
dc.subject.otherLipase
dc.subject.otherOrganic tolerant
dc.subject.otherThermotolerant
dc.subject.otherBacillus
dc.subject.otherBacterial Proteins
dc.subject.otherDetergents
dc.subject.otherHot Temperature
dc.subject.otherLipase
dc.subject.otherSolvents
dc.subject.otherBacillus sp.
dc.subject.otherBacillus
dc.subject.otherDetergent tolerant
dc.subject.otherLipase
dc.subject.otherOrganic tolerant
dc.subject.otherThermotolerant
dc.titlePurification and characterization of organic solvent and detergent tolerant lipase from thermotolerant Bacillus sp. RN2
dc.typeArticle
dspace.entity.typePublication
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