Publication:
Purification and characterization of organic solvent and detergent tolerant lipase from thermotolerant Bacillus sp. RN2

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Issued Date

2010

Resource Type

Article

File Type

application/pdf

ISSN

14220067

DOI

10.3390/ijms11103783

Other identifier(s)

2-s2.0-77958115682

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

International Journal of Molecular Sciences. Vol 11, No.10 (2010), p.3783-3792

Suggested Citation

Kanjanavas P., Khuchareontaworn S., Khawsak P., Pakpitcharoen A., Pothivejkul K., Santiwatanakul S., Matsui K., Kajiwara T., Chansiri K. Purification and characterization of organic solvent and detergent tolerant lipase from thermotolerant Bacillus sp. RN2. International Journal of Molecular Sciences. Vol 11, No.10 (2010), p.3783-3792. doi:10.3390/ijms11103783 Retrieved from: https://hdl.handle.net/20.500.14740/7535

Title

Purification and characterization of organic solvent and detergent tolerant lipase from thermotolerant Bacillus sp. RN2

Author(s)

Kanjanavas P.
 Khuchareontaworn S.
 Khawsak P.
 Pakpitcharoen A.
 Pothivejkul K.
 Santiwatanakul S.
 Matsui K.
 Kajiwara T.
 Chansiri K.

Abstract

The aim of this study was to characterize the organic solvent and detergent tolerant properties of recombinant lipase isolated from thermotolerant Bacillus sp. RN2 (Lip-SBRN2). The isolation of the lipase-coding gene was achieved by the use of inverse and direct PCR. The complete DNA sequencing of the gene revealed that the lip-SBRN2 gene contains 576 nucleotides which corresponded to 192 deduced amino acids. The purified enzyme was homogeneous with the estimated molecular mass of 19 kDa as determined by SDS-PAGE and gel filtration. The Lip-SBRN2 was stable in a pH range of 9-11 and temperature range of 45-60 °C. The enzyme was a non metallo-monomeric protein and was active against pNP-caprylate (C8) and pNP-laurate (C12) and coconut oil. The Lip-SBRN2 exhibited a high level of activity in the presence of 108% benzene, 102.4% diethylether and 112% SDS. It is anticipated that the organic solvent and detergent tolerant enzyme secreted by Bacillus sp. RN2 will be applicable as catalysts for reaction in the presence of organic solvents and detergents. © 2010 by the authors; licensee MDPI.

Subject(s)

4 nitrophenyl caprylate
 Bacterial enzyme
 Benzene
 Coconut oil
 Detergent
 Edetic acid
 Esterase inhibitor
 Ether
 Lauric acid 4 nitrophenyl ester
 Lauric acid derivative
 Metal ion
 Octanoic acid
 Octanoic acid derivative
 Organic solvent
 Recombinant enzyme
 RN2 protein
 Triacylglycerol lipase
 Unclassified drug
 Article
 Bacillus
 Bacterial strain
 Biocatalyst
 Controlled study
 DNA sequence
 Enzyme activity
 Enzyme analysis
 Enzyme isolation
 Enzyme purification
 Enzyme specificity
 Enzyme stability
 Gel filtration
 Genetic code
 Heat tolerance
 Molecular weight
 Nonhuman
 Nucleotide sequence
 PH measurement
 Polyacrylamide gel electrophoresis
 Polymerase chain reaction
 Bacillus
 Detergent tolerant
 Lipase
 Organic tolerant
 Thermotolerant
 Bacillus
 Bacterial Proteins
 Detergents
 Hot Temperature
 Lipase
 Solvents
 Bacillus sp.
 Bacillus
 Detergent tolerant
 Lipase
 Organic tolerant
 Thermotolerant

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URI

https://hdl.handle.net/20.500.14740/7535

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Scopus: Year 1983-2021

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