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Synergistic inhibition of human carcinoma cell growth via co-delivery of p53 plasmid DNA and bcl-2 antisense oligodeoxyribonucleotide by cholic acid-modified polyethylenimine

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dc.contributor.authorWeecharangsan W.
dc.contributor.authorOpanasopit P.
dc.contributor.authorNiyomtham N.
dc.contributor.authorYingyongnarongkul B.-E.
dc.contributor.authorKewsuwan P.
dc.contributor.authorLee R.J.
dc.date.accessioned2021-04-05T03:21:58Z
dc.date.available2021-04-05T03:21:58Z
dc.date.issued2017
dc.date.issuedBE2560
dc.description.abstractBackground/Aim: This study investigated the codelivery of plasmid DNA and antisense oligodeoxyribonucleotide (AS ODN) into carcinoma cells by cholic acidmodified polyethylenimine (PEI-CA). Materials and Methods: PEI-CA/plasmid DNA and AS ODN complexes were formulated and evaluated for delivery of plasmid DNA and AS ODN in HeLa cells. The efficiency of co-delivery of plasmid DNA and AS ODN was evaluated by cell growth inhibition using p53 and bcl-2 AS ODN. Results: AS ODN intracellular delivery and green fluorescent protein expression upon cellular transfection were greater than in cells treated with uncomplexed nucleic acids. Treatment of the cells with PEI-CA/p53 plasmid DNA and bcl-2 AS ODN complexes resulted in cell growth inhibition that was greater than that of either PEI-CA/p53 plasmid DNA complexes or PEI-CA/bcl-2 AS ODN complexes alone. Conclusion: The co-delivery of p53 plasmid DNA and bcl-2 AS ODN in PEI-CA complexes enhanced therapeutic activities of both p53 plasmid DNA and bcl-2 AS ODN.
dc.format.mimetypeapplication/pdf
dc.identifier.citationAnticancer Research. Vol 37, No.11 (2017), p.6335-6340
dc.identifier.doi10.21873/anticanres.12085
dc.identifier.issn2507005
dc.identifier.other2-s2.0-85032161426
dc.identifier.urihttps://hdl.handle.net/20.500.14740/4027
dc.rights.holderมหาวิทยาลัยศรีนครินทรวิโรฒ
dc.subject.otherAntisense oligodeoxynucleotide
dc.subject.otherCholic acid
dc.subject.otherGreen fluorescent protein
dc.subject.otherPlasmid DNA
dc.subject.otherPolyethyleneimine
dc.subject.otherProtein bcl 2
dc.subject.otherProtein p53
dc.subject.otherAntisense oligodeoxynucleotide
dc.subject.otherBCL2 protein, human
dc.subject.otherProtein bcl 2
dc.subject.otherProtein p53
dc.subject.otherAgar gel electrophoresis
dc.subject.otherAntineoplastic activity
dc.subject.otherArticle
dc.subject.otherCancer inhibition
dc.subject.otherCarcinoma cell
dc.subject.otherCell culture
dc.subject.otherCell growth
dc.subject.otherControlled study
dc.subject.otherFluorescence activated cell sorting
dc.subject.otherGenetic transfection
dc.subject.otherGrowth inhibition
dc.subject.otherHuman
dc.subject.otherHuman cell
dc.subject.otherNonviral gene delivery system
dc.subject.otherParticle size
dc.subject.otherPriority journal
dc.subject.otherProtein expression
dc.subject.otherZeta potential
dc.subject.otherAntagonists and inhibitors
dc.subject.otherCarcinoma
dc.subject.otherCell proliferation
dc.subject.otherDrug effects
dc.subject.otherDrug potentiation
dc.subject.otherGene therapy
dc.subject.otherGene vector
dc.subject.otherGenetics
dc.subject.otherHeLa cell line
dc.subject.otherMetabolism
dc.subject.otherPharmacology
dc.subject.otherPlasmid
dc.subject.otherCarcinoma
dc.subject.otherCell Proliferation
dc.subject.otherDrug Synergism
dc.subject.otherGenetic Therapy
dc.subject.otherGenetic Vectors
dc.subject.otherGreen Fluorescent Proteins
dc.subject.otherHeLa Cells
dc.subject.otherHumans
dc.subject.otherOligodeoxyribonucleotides, Antisense
dc.subject.otherPlasmids
dc.subject.otherProto-Oncogene Proteins c-bcl-2
dc.subject.otherTumor Suppressor Protein p53
dc.titleSynergistic inhibition of human carcinoma cell growth via co-delivery of p53 plasmid DNA and bcl-2 antisense oligodeoxyribonucleotide by cholic acid-modified polyethylenimine
dc.typeArticle
dspace.entity.typePublication
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