Publication: Synergistic inhibition of human carcinoma cell growth via co-delivery of p53 plasmid DNA and bcl-2 antisense oligodeoxyribonucleotide by cholic acid-modified polyethylenimine
2
0
Issued Date
2017
Resource Type
File Type
application/pdf
ISSN
2507005
Other identifier(s)
2-s2.0-85032161426
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Anticancer Research. Vol 37, No.11 (2017), p.6335-6340
Suggested Citation
Weecharangsan W., Opanasopit P., Niyomtham N., Yingyongnarongkul B.-E., Kewsuwan P., Lee R.J. Synergistic inhibition of human carcinoma cell growth via co-delivery of p53 plasmid DNA and bcl-2 antisense oligodeoxyribonucleotide by cholic acid-modified polyethylenimine. Anticancer Research. Vol 37, No.11 (2017), p.6335-6340. doi:10.21873/anticanres.12085 Retrieved from: https://hdl.handle.net/20.500.14740/4027
Abstract
Background/Aim: This study investigated the codelivery of plasmid DNA and antisense oligodeoxyribonucleotide (AS ODN) into carcinoma cells by cholic acidmodified polyethylenimine (PEI-CA). Materials and Methods: PEI-CA/plasmid DNA and AS ODN complexes were formulated and evaluated for delivery of plasmid DNA and AS ODN in HeLa cells. The efficiency of co-delivery of plasmid DNA and AS ODN was evaluated by cell growth inhibition using p53 and bcl-2 AS ODN. Results: AS ODN intracellular delivery and green fluorescent protein expression upon cellular transfection were greater than in cells treated with uncomplexed nucleic acids. Treatment of the cells with PEI-CA/p53 plasmid DNA and bcl-2 AS ODN complexes resulted in cell growth inhibition that was greater than that of either PEI-CA/p53 plasmid DNA complexes or PEI-CA/bcl-2 AS ODN complexes alone. Conclusion: The co-delivery of p53 plasmid DNA and bcl-2 AS ODN in PEI-CA complexes enhanced therapeutic activities of both p53 plasmid DNA and bcl-2 AS ODN.
Subject(s)
Antisense oligodeoxynucleotide
Cholic acid
Green fluorescent protein
Plasmid DNA
Polyethyleneimine
Protein bcl 2
Protein p53
Antisense oligodeoxynucleotide
BCL2 protein, human
Protein bcl 2
Protein p53
Agar gel electrophoresis
Antineoplastic activity
Article
Cancer inhibition
Carcinoma cell
Cell culture
Cell growth
Controlled study
Fluorescence activated cell sorting
Genetic transfection
Growth inhibition
Human
Human cell
Nonviral gene delivery system
Particle size
Priority journal
Protein expression
Zeta potential
Antagonists and inhibitors
Carcinoma
Cell proliferation
Drug effects
Drug potentiation
Gene therapy
Gene vector
Genetics
HeLa cell line
Metabolism
Pharmacology
Plasmid
Carcinoma
Cell Proliferation
Drug Synergism
Genetic Therapy
Genetic Vectors
Green Fluorescent Proteins
HeLa Cells
Humans
Oligodeoxyribonucleotides, Antisense
Plasmids
Proto-Oncogene Proteins c-bcl-2
Tumor Suppressor Protein p53
Cholic acid
Green fluorescent protein
Plasmid DNA
Polyethyleneimine
Protein bcl 2
Protein p53
Antisense oligodeoxynucleotide
BCL2 protein, human
Protein bcl 2
Protein p53
Agar gel electrophoresis
Antineoplastic activity
Article
Cancer inhibition
Carcinoma cell
Cell culture
Cell growth
Controlled study
Fluorescence activated cell sorting
Genetic transfection
Growth inhibition
Human
Human cell
Nonviral gene delivery system
Particle size
Priority journal
Protein expression
Zeta potential
Antagonists and inhibitors
Carcinoma
Cell proliferation
Drug effects
Drug potentiation
Gene therapy
Gene vector
Genetics
HeLa cell line
Metabolism
Pharmacology
Plasmid
Carcinoma
Cell Proliferation
Drug Synergism
Genetic Therapy
Genetic Vectors
Green Fluorescent Proteins
HeLa Cells
Humans
Oligodeoxyribonucleotides, Antisense
Plasmids
Proto-Oncogene Proteins c-bcl-2
Tumor Suppressor Protein p53