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Development of a polyclonal antibody specific to VP19 envelope protein of white spot syndrome virus (WSSV) using a recombinant protein preparation

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dc.contributor.authorChaivisuthangkura P.
dc.contributor.authorPhattanapaijitkul P.
dc.contributor.authorThammapalerd N.
dc.contributor.authorRukpratanporn S.
dc.contributor.authorLongyant S.
dc.contributor.authorSithigorngul W.
dc.contributor.authorSithigorngul P.
dc.date.accessioned2021-04-05T04:32:21Z
dc.date.available2021-04-05T04:32:21Z
dc.date.issued2006
dc.date.issuedBE2549
dc.description.abstractThe VP19 gene encoding a structural envelope protein of white spot syndrome virus was cloned into an expression vector and introduced into E. coli. The objective was to produce a recombinant VP19 structural protein. After induction, the recombinant VP19 protein (rVP19) was produced, purified by SDS-PAGE and used for immunization of Swiss mice for polyclonal antibody production. The mouse anti rVP19 antiserum had specific immunoreactivity to the viral antigen in WSSV infected Penaeus monodon as verified by immunohistochemistry and Western blot. The production of monoclonal antibodies against this rVP19 may be useful in order to combine with anti-VP28 monoclonal antibodies for enhancing the sensitivity of various WSSV serological assays. © 2006 Elsevier B.V. All rights reserved.
dc.format.mimetypeapplication/pdf
dc.identifier.citationJournal of Virological Methods. Vol 133, No.2 (2006), p.180-184
dc.identifier.doi10.1016/j.jviromet.2005.11.012
dc.identifier.issn1660934
dc.identifier.other2-s2.0-33645284994
dc.identifier.urihttps://hdl.handle.net/20.500.14740/5733
dc.rights.holderมหาวิทยาลัยศรีนครินทรวิโรฒ
dc.subject.otherEnvelope protein
dc.subject.otherMaltose binding protein
dc.subject.otherMonoclonal antibody
dc.subject.otherPolyclonal antibody
dc.subject.otherProtein vp 19
dc.subject.otherRecombinant protein
dc.subject.otherStructural protein
dc.subject.otherUnclassified drug
dc.subject.otherArticle
dc.subject.otherImmunohistochemistry
dc.subject.otherMouse
dc.subject.otherNonhuman
dc.subject.otherPolyacrylamide gel electrophoresis
dc.subject.otherPolymerase chain reaction
dc.subject.otherPriority journal
dc.subject.otherWestern blotting
dc.subject.otherWhite spot syndrome virus
dc.subject.otherAnimals
dc.subject.otherAntibodies, Monoclonal
dc.subject.otherAntibodies, Viral
dc.subject.otherBlotting, Western
dc.subject.otherDNA, Viral
dc.subject.otherElectrophoresis, Polyacrylamide Gel
dc.subject.otherEscherichia coli
dc.subject.otherEthidium
dc.subject.otherGenes, Viral
dc.subject.otherImmunohistochemistry
dc.subject.otherMice
dc.subject.otherPenaeidae
dc.subject.otherPlasmids
dc.subject.otherRecombinant Proteins
dc.subject.otherViral Envelope Proteins
dc.subject.otherVirology
dc.subject.otherWhite spot syndrome virus 1
dc.subject.otherPenaeus monodon
dc.subject.otherShrimp white spot syndrome virus
dc.titleDevelopment of a polyclonal antibody specific to VP19 envelope protein of white spot syndrome virus (WSSV) using a recombinant protein preparation
dc.typeArticle
dspace.entity.typePublication
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