Publication:
Development of a polyclonal antibody specific to VP19 envelope protein of white spot syndrome virus (WSSV) using a recombinant protein preparation

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Issued Date

2006

Resource Type

Article

File Type

application/pdf

ISSN

1660934

DOI

10.1016/j.jviromet.2005.11.012

Other identifier(s)

2-s2.0-33645284994

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Virological Methods. Vol 133, No.2 (2006), p.180-184

Suggested Citation

Chaivisuthangkura P., Phattanapaijitkul P., Thammapalerd N., Rukpratanporn S., Longyant S., Sithigorngul W., Sithigorngul P. Development of a polyclonal antibody specific to VP19 envelope protein of white spot syndrome virus (WSSV) using a recombinant protein preparation. Journal of Virological Methods. Vol 133, No.2 (2006), p.180-184. doi:10.1016/j.jviromet.2005.11.012 Retrieved from: https://hdl.handle.net/20.500.14740/5733

Title

Development of a polyclonal antibody specific to VP19 envelope protein of white spot syndrome virus (WSSV) using a recombinant protein preparation

Author(s)

Chaivisuthangkura P.
 Phattanapaijitkul P.
 Thammapalerd N.
 Rukpratanporn S.
 Longyant S.
 Sithigorngul W.
 Sithigorngul P.

Abstract

The VP19 gene encoding a structural envelope protein of white spot syndrome virus was cloned into an expression vector and introduced into E. coli. The objective was to produce a recombinant VP19 structural protein. After induction, the recombinant VP19 protein (rVP19) was produced, purified by SDS-PAGE and used for immunization of Swiss mice for polyclonal antibody production. The mouse anti rVP19 antiserum had specific immunoreactivity to the viral antigen in WSSV infected Penaeus monodon as verified by immunohistochemistry and Western blot. The production of monoclonal antibodies against this rVP19 may be useful in order to combine with anti-VP28 monoclonal antibodies for enhancing the sensitivity of various WSSV serological assays. © 2006 Elsevier B.V. All rights reserved.

Subject(s)

Envelope protein
 Maltose binding protein
 Monoclonal antibody
 Polyclonal antibody
 Protein vp 19
 Recombinant protein
 Structural protein
 Unclassified drug
 Article
 Immunohistochemistry
 Mouse
 Nonhuman
 Polyacrylamide gel electrophoresis
 Polymerase chain reaction
 Priority journal
 Western blotting
 White spot syndrome virus
 Animals
 Antibodies, Monoclonal
 Antibodies, Viral
 Blotting, Western
 DNA, Viral
 Electrophoresis, Polyacrylamide Gel
 Escherichia coli
 Ethidium
 Genes, Viral
 Immunohistochemistry
 Mice
 Penaeidae
 Plasmids
 Recombinant Proteins
 Viral Envelope Proteins
 Virology
 White spot syndrome virus 1
 Penaeus monodon
 Shrimp white spot syndrome virus

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URI

https://hdl.handle.net/20.500.14740/5733

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Scopus: Year 1983-2021

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