Publication:
Lack of specificity of commercially available antisera against muscarinergic and adrenergic receptors

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dc.contributor.authorPradidarcheep W.
dc.contributor.authorStallen J.
dc.contributor.authorLabruyère W.T.
dc.contributor.authorDabhoiwala N.F.
dc.contributor.authorMichel M.C.
dc.contributor.authorLamers W.H.
dc.date.accessioned2021-04-05T04:33:31Z
dc.date.available2021-04-05T04:33:31Z
dc.date.issued2009
dc.date.issuedBE2552
dc.description.abstractCommercially available antisera against five subtypes of muscarinic receptors and nine subtypes of adrenoceptors showed highly distinct immunohistochemical staining patterns in rat ureter and stomach. However, using the M1-4 muscarinic receptor subtypes and α2B-, β2-, and β3-adrenoceptors as examples, Western blots with membranes prepared from cell lines stably expressing various subtypes of muscarinic receptors or adrenoceptors revealed that each of the antisera recognized a set of proteins that differed between the cell lines used but lacked specificity for the claimed target receptor. We propose that receptor antibodies need better validation before they can reliably be used.
dc.format.mimetypeapplication/pdf
dc.identifier.citationNaunyn-Schmiedeberg's Archives of Pharmacology. Vol 379, No.4 (2009), p.397-402
dc.identifier.doi10.1007/s00210-009-0393-0
dc.identifier.issn281298
dc.identifier.other2-s2.0-60849088554
dc.identifier.urihttps://hdl.handle.net/20.500.14740/7157
dc.rights.holderมหาวิทยาลัยศรีนครินทรวิโรฒ
dc.subject.otherAlpha 2B adrenergic receptor
dc.subject.otherAlpha 2b adrenergic receptor antibody
dc.subject.otherAntiserum
dc.subject.otherBeta 2 adrenergic receptor
dc.subject.otherBeta 2 adrenergic receptor antibody
dc.subject.otherMuscarinic M1 receptor
dc.subject.otherMuscarinic m1 receptor antibody
dc.subject.otherMuscarinic M2 receptor
dc.subject.otherMuscarinic m2 receptor antibody
dc.subject.otherMuscarinic M3 receptor
dc.subject.otherMuscarinic m3 receptor antibody
dc.subject.otherMuscarinic M4 receptor
dc.subject.otherMuscarinic m4 receptor antibody
dc.subject.otherReceptor antibody
dc.subject.otherUnclassified drug
dc.subject.otherAnimal cell
dc.subject.otherAntibody specificity
dc.subject.otherAntigen recognition
dc.subject.otherArticle
dc.subject.otherCell membrane
dc.subject.otherHuman
dc.subject.otherHuman cell
dc.subject.otherImmunohistochemistry
dc.subject.otherNonhuman
dc.subject.otherProtein expression
dc.subject.otherRat
dc.subject.otherWestern blotting
dc.subject.otherAnimals
dc.subject.otherAntibodies
dc.subject.otherAntibody Specificity
dc.subject.otherBlotting, Western
dc.subject.otherBrain Chemistry
dc.subject.otherCell Line
dc.subject.otherGastrointestinal Tract
dc.subject.otherHumans
dc.subject.otherImmune Sera
dc.subject.otherImmunohistochemistry
dc.subject.otherRats
dc.subject.otherRats, Wistar
dc.subject.otherReceptors, Adrenergic
dc.subject.otherReceptors, Muscarinic
dc.subject.otherTransfection
dc.subject.otherUrinary Tract
dc.titleLack of specificity of commercially available antisera against muscarinergic and adrenergic receptors
dc.typeArticle
dspace.entity.typePublication
swu.datasource.scopushttps://www.scopus.com/inward/record.uri?eid=2-s2.0-60849088554&doi=10.1007%2fs00210-009-0393-0&partnerID=40&md5=d0e8080079d633313ad4db818bf0a295

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