Publication:
Lack of specificity of commercially available antisera against muscarinergic and adrenergic receptors

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Issued Date

2009

Resource Type

Article

File Type

application/pdf

ISSN

281298

DOI

10.1007/s00210-009-0393-0

Other identifier(s)

2-s2.0-60849088554

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Naunyn-Schmiedeberg's Archives of Pharmacology. Vol 379, No.4 (2009), p.397-402

Suggested Citation

Pradidarcheep W., Stallen J., Labruyère W.T., Dabhoiwala N.F., Michel M.C., Lamers W.H. Lack of specificity of commercially available antisera against muscarinergic and adrenergic receptors. Naunyn-Schmiedeberg's Archives of Pharmacology. Vol 379, No.4 (2009), p.397-402. doi:10.1007/s00210-009-0393-0 Retrieved from: https://hdl.handle.net/20.500.14740/7157

Title

Lack of specificity of commercially available antisera against muscarinergic and adrenergic receptors

Author(s)

Pradidarcheep W.
 Stallen J.
 Labruyère W.T.
 Dabhoiwala N.F.
 Michel M.C.
 Lamers W.H.

Abstract

Commercially available antisera against five subtypes of muscarinic receptors and nine subtypes of adrenoceptors showed highly distinct immunohistochemical staining patterns in rat ureter and stomach. However, using the M1-4 muscarinic receptor subtypes and α2B-, β2-, and β3-adrenoceptors as examples, Western blots with membranes prepared from cell lines stably expressing various subtypes of muscarinic receptors or adrenoceptors revealed that each of the antisera recognized a set of proteins that differed between the cell lines used but lacked specificity for the claimed target receptor. We propose that receptor antibodies need better validation before they can reliably be used.

Subject(s)

Alpha 2B adrenergic receptor
 Alpha 2b adrenergic receptor antibody
 Antiserum
 Beta 2 adrenergic receptor
 Beta 2 adrenergic receptor antibody
 Muscarinic M1 receptor
 Muscarinic m1 receptor antibody
 Muscarinic M2 receptor
 Muscarinic m2 receptor antibody
 Muscarinic M3 receptor
 Muscarinic m3 receptor antibody
 Muscarinic M4 receptor
 Muscarinic m4 receptor antibody
 Receptor antibody
 Unclassified drug
 Animal cell
 Antibody specificity
 Antigen recognition
 Article
 Cell membrane
 Human
 Human cell
 Immunohistochemistry
 Nonhuman
 Protein expression
 Rat
 Western blotting
 Animals
 Antibodies
 Antibody Specificity
 Blotting, Western
 Brain Chemistry
 Cell Line
 Gastrointestinal Tract
 Humans
 Immune Sera
 Immunohistochemistry
 Rats
 Rats, Wistar
 Receptors, Adrenergic
 Receptors, Muscarinic
 Transfection
 Urinary Tract

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URI

https://hdl.handle.net/20.500.14740/7157

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Scopus: Year 1983-2021

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