Publication: A new alternative assay for sensitive analysis of ethylenethiourea and propylenethiourea in fruit samples after their separation
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Issued Date
2020
Resource Type
File Type
application/pdf
ISSN
17599660
Other identifier(s)
2-s2.0-85089505220
Rights Holder(s)
Scopus
Bibliographic Citation
Analytical Methods. Vol 12, No.29 (2020), p.3705-3712
Suggested Citation
Nantaphol S., Moonla C., Promvichai S., Tangkuaram T., Chailapakul O., Siangproh W. A new alternative assay for sensitive analysis of ethylenethiourea and propylenethiourea in fruit samples after their separation. Analytical Methods. Vol 12, No.29 (2020), p.3705-3712. doi:10.1039/d0ay01001d Retrieved from: https://hdl.handle.net/20.500.14740/4431
Abstract
Ultra-high-performance liquid chromatography (UHPLC) coupled with a cobalt phthalocyanine screen-printed carbon electrode (CoPc-SPCE) was developed and validated for quantitative analysis of ethylenethiourea (ETU) and propylenethiourea (PTU). CoPC-SPCE provided high catalytic properties for ETU and PTU oxidation. This fabricated electrode is inexpensive, disposable, and easy to prepare by an in-house screen-printing technique. The chromatographic separation was performed in isocratic mode on a reversed phase C18 (100 mm × 4.6 mm, 3 µm) column, using a 90?:?10 (v/v) ratio of 0.05 M phosphate buffer solution (pH 4) and methanol as the mobile phase with a flow rate of 1.0 mL min-1 at an oxidation potential of +0.7 V vs. Ag/AgCl. The separation could be achieved within 3 min, and a wide linear range of 0.01-100 µg mL-1 (r2 > 0.99) was obtained for both analytes. The limits of detection (3 S/N) were found to be 0.006 and 0.009 µg mL-1 for ETU and PTU, respectively. Furthermore, this proposed method was utilized to determine ETU and PTU in fruit samples with satisfactory results, yielding excellent intra-day and inter-day relative standard deviations and recoveries. These results demonstrated that the proposed assay can be used as a new alternative way for inexpensive, rapid, selective and sensitive determination of ETU and PTU in fruit samples. © The Royal Society of Chemistry.
Subject(s)
Catalytic oxidation
Electrodes
High performance liquid chromatography
High pressure liquid chromatography
Screen printing
Chromatographic separations
Cobalt phthalocyanine
Phosphate buffer solutions
Propylenethiourea (PTU)
Relative standard deviations
Screen printing technique
Screen-printed carbon electrodes
Ultra high performance liquid chromatography (UHPLC)
Fruits
Electrodes
High performance liquid chromatography
High pressure liquid chromatography
Screen printing
Chromatographic separations
Cobalt phthalocyanine
Phosphate buffer solutions
Propylenethiourea (PTU)
Relative standard deviations
Screen printing technique
Screen-printed carbon electrodes
Ultra high performance liquid chromatography (UHPLC)
Fruits