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Genetic diversity of Trypanosoma evansi in beef cattle based on internal transcribed spacer region

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dc.contributor.authorAreekit S.
dc.contributor.authorSinghaphan P.
dc.contributor.authorKanjanavas P.
dc.contributor.authorKhuchareontaworn S.
dc.contributor.authorSriyapai T.
dc.contributor.authorPakpitcharoen A.
dc.contributor.authorChansiri K.
dc.date.accessioned2021-04-05T04:31:58Z
dc.date.available2021-04-05T04:31:58Z
dc.date.issued2008
dc.date.issuedBE2551
dc.description.abstractThis study was focused on genetic diversity of Trypanosoma evansi which is a widely distributed haemoflagellate of veterinary importance that infects a variety of larger mammals including horses, mules, camels, buffalo, cattle and deer. The genetic diversity of T. evansi of beef cattle LAM19 was accomplished by using phylogenetic analysis based on internal transcribed spacer region (ITS). Blood sample was collected from a naturally infected beef cattle LAM 19 and parasitemia was raised by mouse inoculation. The parasites were collected and isolated by using DE 52 DEAE cellulose anion exchange column prior to DNA extraction. Upon PCR amplification of ITS region, the product of 1300 bp in size was obtained. The ITS nucleotide sequences were analyzed and revealed that it could demonstrate the genetic diversity of T. evansi of beef cattle LAM19. Based on the ITS tree, beef cattle LAM 19 T. evansi were categorized into two main groups where the genetic diversity occurred within Group 1. The data could be applicable for the survey of parasite dynamics, epidemiological studies as well as prevention and control of the disease. © 2007 Elsevier B.V. All rights reserved.
dc.format.mimetypeapplication/pdf
dc.identifier.citationInfection, Genetics and Evolution. Vol 8, No.4 (2008), p.484-488
dc.identifier.doi10.1016/j.meegid.2007.10.008
dc.identifier.issn15671348
dc.identifier.other2-s2.0-43649089607
dc.identifier.urihttps://hdl.handle.net/20.500.14740/3946
dc.rights.holderScopus
dc.subject.otherCellulose
dc.subject.otherInternal transcribed spacer
dc.subject.otherAnion exchange
dc.subject.otherArticle
dc.subject.otherBeef
dc.subject.otherBlood sampling
dc.subject.otherCattle
dc.subject.otherDisease control
dc.subject.otherGene amplification
dc.subject.otherGenetic variability
dc.subject.otherMolecular cloning
dc.subject.otherNonhuman
dc.subject.otherNucleotide sequence
dc.subject.otherParasite isolation
dc.subject.otherParasitemia
dc.subject.otherPhylogeny
dc.subject.otherPriority journal
dc.subject.otherProtozoal genetics
dc.subject.otherTrypanosoma evansi
dc.subject.otherAnimals
dc.subject.otherCattle
dc.subject.otherDNA, Intergenic
dc.subject.otherDNA, Protozoan
dc.subject.otherMolecular Sequence Data
dc.subject.otherPhylogeny
dc.subject.otherTrypanosoma
dc.subject.otherTrypanosomiasis, Bovine
dc.subject.otherVariation (Genetics)
dc.subject.otherBos
dc.subject.otherCamelidae
dc.subject.otherCervidae
dc.subject.otherEquidae
dc.subject.otherMammalia
dc.subject.otherTrypanosoma evansi
dc.titleGenetic diversity of Trypanosoma evansi in beef cattle based on internal transcribed spacer region
dc.typeArticle
dspace.entity.typePublication
swu.datasource.scopushttps://www.scopus.com/inward/record.uri?eid=2-s2.0-43649089607&doi=10.1016%2fj.meegid.2007.10.008&partnerID=40&md5=5bd2fc63e5e3091bf889b229cffb457a

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