Publication: Genetic diversity of Trypanosoma evansi in beef cattle based on internal transcribed spacer region
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Issued Date
2008
Resource Type
File Type
application/pdf
ISSN
15671348
Other identifier(s)
2-s2.0-43649089607
Rights Holder(s)
Scopus
Bibliographic Citation
Infection, Genetics and Evolution. Vol 8, No.4 (2008), p.484-488
Suggested Citation
Areekit S., Singhaphan P., Kanjanavas P., Khuchareontaworn S., Sriyapai T., Pakpitcharoen A., Chansiri K. Genetic diversity of Trypanosoma evansi in beef cattle based on internal transcribed spacer region. Infection, Genetics and Evolution. Vol 8, No.4 (2008), p.484-488. doi:10.1016/j.meegid.2007.10.008 Retrieved from: https://hdl.handle.net/20.500.14740/3946
Abstract
This study was focused on genetic diversity of Trypanosoma evansi which is a widely distributed haemoflagellate of veterinary importance that infects a variety of larger mammals including horses, mules, camels, buffalo, cattle and deer. The genetic diversity of T. evansi of beef cattle LAM19 was accomplished by using phylogenetic analysis based on internal transcribed spacer region (ITS). Blood sample was collected from a naturally infected beef cattle LAM 19 and parasitemia was raised by mouse inoculation. The parasites were collected and isolated by using DE 52 DEAE cellulose anion exchange column prior to DNA extraction. Upon PCR amplification of ITS region, the product of 1300 bp in size was obtained. The ITS nucleotide sequences were analyzed and revealed that it could demonstrate the genetic diversity of T. evansi of beef cattle LAM19. Based on the ITS tree, beef cattle LAM 19 T. evansi were categorized into two main groups where the genetic diversity occurred within Group 1. The data could be applicable for the survey of parasite dynamics, epidemiological studies as well as prevention and control of the disease. © 2007 Elsevier B.V. All rights reserved.
Subject(s)
Cellulose
Internal transcribed spacer
Anion exchange
Article
Beef
Blood sampling
Cattle
Disease control
Gene amplification
Genetic variability
Molecular cloning
Nonhuman
Nucleotide sequence
Parasite isolation
Parasitemia
Phylogeny
Priority journal
Protozoal genetics
Trypanosoma evansi
Animals
Cattle
DNA, Intergenic
DNA, Protozoan
Molecular Sequence Data
Phylogeny
Trypanosoma
Trypanosomiasis, Bovine
Variation (Genetics)
Bos
Camelidae
Cervidae
Equidae
Mammalia
Trypanosoma evansi
Internal transcribed spacer
Anion exchange
Article
Beef
Blood sampling
Cattle
Disease control
Gene amplification
Genetic variability
Molecular cloning
Nonhuman
Nucleotide sequence
Parasite isolation
Parasitemia
Phylogeny
Priority journal
Protozoal genetics
Trypanosoma evansi
Animals
Cattle
DNA, Intergenic
DNA, Protozoan
Molecular Sequence Data
Phylogeny
Trypanosoma
Trypanosomiasis, Bovine
Variation (Genetics)
Bos
Camelidae
Cervidae
Equidae
Mammalia
Trypanosoma evansi