Publication:
Effects of melatonin on severe crush spinal cord injury–induced reactive astrocyte and scar formation

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Issued Date

2016

Resource Type

Article

File Type

application/pdf

ISSN

3604012

DOI

10.1002/jnr.23930

Other identifier(s)

2-s2.0-84988905763

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Neuroscience Research. Vol 94, No.12 (2016), p.1451-1459

Suggested Citation

Krityakiarana W., Sompup K., Jongkamonwiwat N., Mukda S., Pinilla F.G., Govitrapong P., Phansuwan-Pujito P. Effects of melatonin on severe crush spinal cord injury–induced reactive astrocyte and scar formation. Journal of Neuroscience Research. Vol 94, No.12 (2016), p.1451-1459. doi:10.1002/jnr.23930 Retrieved from: https://hdl.handle.net/20.500.14740/4908

Title

Effects of melatonin on severe crush spinal cord injury–induced reactive astrocyte and scar formation

Author(s)

Krityakiarana W.
 Sompup K.
 Jongkamonwiwat N.
 Mukda S.
 Pinilla F.G.
 Govitrapong P.
 Phansuwan-Pujito P.

Abstract

The present work aimed at analyzing the effects of melatonin on scar formation after spinal cord injury (SCI). Upregulation of reactive astrocyte under SCI pathological conditions has been presented in several studies. It has been proved that the crucial factor in triggering this upregulation is proinflammatory cytokines. Moreover, scar formation is an important barrier to axonal regeneration through the lesion area. Melatonin plays an important role in reducing inflammation, but its effects on scar formation in the injured spinal cord remain unknown. Hence, we used the model of severe crush injury in mice to investigate the effects of melatonin on scar formation. Mice were randomly separated into four groups; SCI, SCI+Melatonin 1 (single dose), SCI+Melatonin 14 (14 daily doses), and control. Melatonin was administered by intraperitoneal injection (10 mg/kg) after injury. Immunohistochemical analysis, Western blot, and behavioral evaluation were used to explore the effects of melatonin after SCI for 14 days. The melatonin-treated mice presented higher expression of neuronal markers (P < 0.001). Remarkably, the inflammatory response appeared to be greatly reduced in the SCI+Melatonin 14 group (P < 0.001), which also displayed less scar formation (P < 0.05). These findings suggest that melatonin inhibits scar formation by acting on inflammatory cytokines after SCI. Overall, our results suggest that melatonin is a promising treatment strategy after SCI that deserves further investigation. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Subject(s)

Cytokine
 Melatonin
 Melatonin
 Neuroprotective agent
 Nonsteroid antiinflammatory agent
 Animal cell
 Animal experiment
 Animal model
 Animal tissue
 Article
 Astrocyte
 Controlled study
 Disease control
 Disease severity
 Drug effect
 Drug efficacy
 Female
 Immunohistochemistry
 Mouse
 Nerve crush
 Nervous system inflammation
 Nonhuman
 Outcome assessment
 Priority journal
 Randomized controlled trial
 Scar formation
 Single drug dose
 Spinal cord injury
 Treatment response
 Western blotting
 Animal
 Animal behavior
 Astrocyte
 Convalescence
 Drug effects
 Locomotion
 Metabolism
 Nerve crush
 Pathology
 Psychology
 Scar
 Spinal cord injury
 Animals
 Anti-Inflammatory Agents, Non-Steroidal
 Astrocytes
 Behavior, Animal
 Cicatrix
 Female
 Locomotion
 Melatonin
 Mice
 Nerve Crush
 Neuroprotective Agents
 Recovery of Function
 Spinal Cord Injuries

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URI

https://hdl.handle.net/20.500.14740/4908

Collections

Scopus: Year 1983-2021

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