Publication:
Disulfide-linked liposomes: Effective delivery vehicle for Bcl-2 antisense oligodeoxyribonucleotide G3139

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Issued Date

2010

Resource Type

Article

File Type

application/pdf

ISSN

2507005

Other identifier(s)

2-s2.0-77649246832

Rights Holder(s)

Scopus

Bibliographic Citation

Anticancer Research. Vol 30, No.1 (2010), p.31-38

Suggested Citation

Weecharangsan W., Yu B., Liu S., Pang J.X., Lee L.J., Marcucci G., Lee R.J. Disulfide-linked liposomes: Effective delivery vehicle for Bcl-2 antisense oligodeoxyribonucleotide G3139. Anticancer Research. Vol 30, No.1 (2010), p.31-38. Retrieved from: https://hdl.handle.net/20.500.14740/7666

Title

Disulfide-linked liposomes: Effective delivery vehicle for Bcl-2 antisense oligodeoxyribonucleotide G3139

Author(s)

Weecharangsan W.
 Yu B.
 Liu S.
 Pang J.X.
 Lee L.J.
 Marcucci G.
 Lee R.J.

Abstract

Background: Disulfide-linked oligodeoxyribonucleotide (ODN) liposomes were formulated and evaluated for the delivery of antisense ODN G3139 in KB human oral carcinoma cells. Materials and Methods: Liposomes composed of 1,2-di-(9Z-octadecenoyl)-3-trimethylammo-nium-propane(DOTAP)/egg phosphatidylcholine/α-tocopheryl polyethylene glycol 1000 succinate were incorporated with hydrophobized disulfide-linked ODN. Disulfide-linked ODN liposomes were characterized for their size, ODN intracellular delivery, Bcl-2 mRNA and protein expression, growth inhibition, and chemosensitization. Results: Intracellular delivery of ODN with disulfide-linked ODN liposomes was more efficient than that with non-liposomal hydrophobized disulfide-linked ODN. Treatment of the cells with disulfide-linked ODN liposomes resulted in efficient Bcl-2 down-regulation greater than that with hydrophobized disulfide-linked ODN and consistent with that of cellular growth inhibition and the sensitization to daunorubicin in KB cells. Disulfide-linked ODN liposomes exhibited superior colloidal stability during 5-week storage. Conclusion: Disulfide-linked liposomes are effective delivery vehicles for antisense ODN.

Subject(s)

1,2 dioleoyl 3 trimethylammoniopropane
 Daunorubicin
 Disulfide linked liposome
 Liposome
 Macrogol derivative
 Messenger RNA
 Oblimersen
 Phosphatidylcholine
 Polyethylene glycol 1000 succinate
 Protein bcl 2
 Unclassified drug
 Article
 Cell growth
 Cell strain KB
 Chemosensitization
 Controlled study
 Down regulation
 Growth inhibition
 Human
 Human cell
 Intracellular transport
 Mouth carcinoma
 Nonviral gene delivery system
 Priority journal
 Protein expression
 Antibiotics, Antineoplastic
 Cell Growth Processes
 Colloids
 Daunorubicin
 Disulfides
 Down-Regulation
 Drug Stability
 Genes, bcl-2
 Humans
 KB Cells
 Liposomes
 Oligodeoxyribonucleotides, Antisense
 Transfection

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URI

https://hdl.handle.net/20.500.14740/7666

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Scopus: Year 1983-2021

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