Publication: Genomic characterisation of Lacticaseibacillus paracasei phage ΦT25 and preliminary analysis of its derived endolysin
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Issued Date
2021
Resource Type
File Type
application/pdf
ISSN
9586946
Other identifier(s)
2-s2.0-85099288738
Rights
Srinakharinwirot University
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
International Dairy Journal. Vol 116, (2021)
Suggested Citation
Sunthornthummas S., Doi K., Fujino Y., Rangsiruji A., Sarawaneeyaruk S., Insian K., Pringsulaka O. Genomic characterisation of Lacticaseibacillus paracasei phage ΦT25 and preliminary analysis of its derived endolysin. International Dairy Journal. Vol 116, (2021). doi:10.1016/j.idairyj.2020.104968 Retrieved from: https://hdl.handle.net/20.500.14740/4665
Abstract
Bacteriophage infection is a serious problem in milk fermentation where it causes a delay in the fermentation process and reduces product quality. This study provides the first molecular characterisation of a lactic acid bacteriophage from spoiled fermented milk tanks in the dairy industry in Thailand. Bacteriophage ΦT25 is virulent, and shows strong lytic activity against Lacticaseibacillus paracasei LPC. Genome analysis demonstrated that phage ΦT25 possesses a linear, double-stranded DNA genome of 38,421 bp. To assay endolysin activity of phage ΦT25 (ORF35), Lysin vector pET21a was constructed by cloning the LysinT25 gene into plasmid pET21a. The size of LysinT25 endolysin was predicted to be ∼41 kDa LysinT25 was able to lyse a wide range of lactic acid bacteria and other Gram-positive bacteria, such as Staphylococcus aureus and Listeria monocytogenes. In addition, this endolysin has a synergistic inhibitory effect against Escherichia coli when combined with chloroform as an outer-membrane permeabiliser. © 2020 Elsevier Ltd
Subject(s)
Bacteriophages
Chlorine compounds
Clone cells
Dairies
Escherichia coli
Fermentation
Fermented milk
Genes
Listeria
Double stranded DNA
Fermentation process
Gram-positive bacterium
Inhibitory effect
Lactic acid bacteria
Listeria monocytogenes
Molecular characterisation
Preliminary analysis
Lactic acid
Chlorine compounds
Clone cells
Dairies
Escherichia coli
Fermentation
Fermented milk
Genes
Listeria
Double stranded DNA
Fermentation process
Gram-positive bacterium
Inhibitory effect
Lactic acid bacteria
Listeria monocytogenes
Molecular characterisation
Preliminary analysis
Lactic acid