Quartz crystal microbalance DNA based biosensor for the detection of brugia malayi

Abstract

This Lymphatic filariasis is the major public health problem caused by Brugia spp. by the conventional methods as PCR cannot discriminate well, takes a few days or several hours. This study demonstrates a sensitive and specific quartz crystal microbalance (QCM) biosensor combined with polymerase chain reaction (PCR) for diagnosis of Brugia malayi. The procedure concludes: formation of self-assembled monolayer (SAM) on gold quartz crystals surface, attachment of the avidin to activated carboxyl groups by EDC/NHS, attachment of the biotin-modified probe to the avidin, and hybridization of probe to the target PCR. The PCR-QCM biosensor system was more specific than a PCR-gel electrophoresis assay in detecting the DNA of B. malayi and B. pahangi. © 2012 IFSA.