Publication: Quartz crystal microbalance DNA based biosensor for the detection of brugia malayi
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Issued Date
2012
Resource Type
File Type
application/pdf
ISSN
17265479
Other identifier(s)
2-s2.0-84873974320
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Sensors and Transducers. Vol 144, No.9 (2012), p.153-160
Suggested Citation
Kaewphinit T., Santiwatanakul S., Areekit S., Chansiri K. Quartz crystal microbalance DNA based biosensor for the detection of brugia malayi. Sensors and Transducers. Vol 144, No.9 (2012), p.153-160. Retrieved from: https://hdl.handle.net/20.500.14740/6853
Author(s)
Abstract
This Lymphatic filariasis is the major public health problem caused by Brugia spp. by the conventional methods as PCR cannot discriminate well, takes a few days or several hours. This study demonstrates a sensitive and specific quartz crystal microbalance (QCM) biosensor combined with polymerase chain reaction (PCR) for diagnosis of Brugia malayi. The procedure concludes: formation of self-assembled monolayer (SAM) on gold quartz crystals surface, attachment of the avidin to activated carboxyl groups by EDC/NHS, attachment of the biotin-modified probe to the avidin, and hybridization of probe to the target PCR. The PCR-QCM biosensor system was more specific than a PCR-gel electrophoresis assay in detecting the DNA of B. malayi and B. pahangi. © 2012 IFSA.