Publication:
Detection of lymphatic Wuchereria bancrofti in carriers and long-term storage blood samples using semi-nested PCR

Simple item page
dc.contributor.authorKanjanavas P.
dc.contributor.authorTan-Ariya P.
dc.contributor.authorKhawsak P.
dc.contributor.authorPakpitcharoen A.
dc.contributor.authorPhantana S.
dc.contributor.authorChansiri K.
dc.date.accessioned2021-04-05T04:32:42Z
dc.date.available2021-04-05T04:32:42Z
dc.date.issued2005
dc.date.issuedBE2548
dc.description.abstractThe semi-nested PCR was conducted for detection of Wuchereria bancrofti in patients' blood. The primers were designed based on the repetitive DNA sequences of the parasite. The results demonstrated that the semi-nested PCR could detect as little as 0.001 fg of parasite DNA. In addition, the primers showed no PCR amplification from human and other hemoparasites such as Brugia malayi, Plasmodium falciparum and P. vivax DNAs. This technique was used for detection of 18 W. bancrofti infected blood samples with a long-term storage, the data revealed that all samples were positive. The results obtained from this study clearly indicated that the semi-nested PCR is specific, sensitive, and suitable for detection of the disease carriers. © 2004 Elsevier Ltd. All rights reserved.
dc.format.mimetypeapplication/pdf
dc.identifier.citationMolecular and Cellular Probes. Vol 19, No.3 (2005), p.169-172
dc.identifier.doi10.1016/j.mcp.2004.11.003
dc.identifier.issn8908508
dc.identifier.other2-s2.0-15944415828
dc.identifier.urihttps://hdl.handle.net/20.500.14740/6313
dc.rights.holderScopus
dc.subject.otherDNA
dc.subject.otherSelfish dna
dc.subject.otherUnclassified drug
dc.subject.otherAccuracy
dc.subject.otherArticle
dc.subject.otherBlood analysis
dc.subject.otherBlood storage
dc.subject.otherBrugia malayi
dc.subject.otherControlled study
dc.subject.otherDNA determination
dc.subject.otherDNA sequence
dc.subject.otherGene amplification
dc.subject.otherNonhuman
dc.subject.otherParasite detection
dc.subject.otherParasite examination
dc.subject.otherPlasmodium falciparum
dc.subject.otherPlasmodium vivax
dc.subject.otherPolymerase chain reaction
dc.subject.otherPriority journal
dc.subject.otherSensitivity and specificity
dc.subject.otherWuchereria bancrofti
dc.subject.otherBrugia malayi
dc.subject.otherPlasmodium falciparum
dc.subject.otherPlasmodium vivax
dc.subject.otherWuchereria bancrofti
dc.titleDetection of lymphatic Wuchereria bancrofti in carriers and long-term storage blood samples using semi-nested PCR
dc.typeArticle
dspace.entity.typePublication
swu.datasource.scopushttps://www.scopus.com/inward/record.uri?eid=2-s2.0-15944415828&doi=10.1016%2fj.mcp.2004.11.003&partnerID=40&md5=19f7ad9eb19c96dc3ee332082614c866

Files

Collections

Scopus: Year 1983-2021