Publication:
Detection of lymphatic Wuchereria bancrofti in carriers and long-term storage blood samples using semi-nested PCR

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Issued Date

2005

Resource Type

Article

File Type

application/pdf

ISSN

8908508

DOI

10.1016/j.mcp.2004.11.003

Other identifier(s)

2-s2.0-15944415828

Rights Holder(s)

Scopus

Bibliographic Citation

Molecular and Cellular Probes. Vol 19, No.3 (2005), p.169-172

Suggested Citation

Kanjanavas P., Tan-Ariya P., Khawsak P., Pakpitcharoen A., Phantana S., Chansiri K. Detection of lymphatic Wuchereria bancrofti in carriers and long-term storage blood samples using semi-nested PCR. Molecular and Cellular Probes. Vol 19, No.3 (2005), p.169-172. doi:10.1016/j.mcp.2004.11.003 Retrieved from: https://hdl.handle.net/20.500.14740/6313

Title

Detection of lymphatic Wuchereria bancrofti in carriers and long-term storage blood samples using semi-nested PCR

Author(s)

Kanjanavas P.
 Tan-Ariya P.
 Khawsak P.
 Pakpitcharoen A.
 Phantana S.
 Chansiri K.

Abstract

The semi-nested PCR was conducted for detection of Wuchereria bancrofti in patients' blood. The primers were designed based on the repetitive DNA sequences of the parasite. The results demonstrated that the semi-nested PCR could detect as little as 0.001 fg of parasite DNA. In addition, the primers showed no PCR amplification from human and other hemoparasites such as Brugia malayi, Plasmodium falciparum and P. vivax DNAs. This technique was used for detection of 18 W. bancrofti infected blood samples with a long-term storage, the data revealed that all samples were positive. The results obtained from this study clearly indicated that the semi-nested PCR is specific, sensitive, and suitable for detection of the disease carriers. © 2004 Elsevier Ltd. All rights reserved.

Subject(s)

DNA
 Selfish dna
 Unclassified drug
 Accuracy
 Article
 Blood analysis
 Blood storage
 Brugia malayi
 Controlled study
 DNA determination
 DNA sequence
 Gene amplification
 Nonhuman
 Parasite detection
 Parasite examination
 Plasmodium falciparum
 Plasmodium vivax
 Polymerase chain reaction
 Priority journal
 Sensitivity and specificity
 Wuchereria bancrofti
 Brugia malayi
 Plasmodium falciparum
 Plasmodium vivax
 Wuchereria bancrofti

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URI

https://hdl.handle.net/20.500.14740/6313

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Scopus: Year 1983-2021

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