Publication: Bile salt hydrolase activity and cholesterol assimilation of lactic acid bacteria isolated from flowers
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Issued Date
2019
Resource Type
File Type
application/pdf
ISSN
22313354
Other identifier(s)
2-s2.0-85067118904
Rights Holder(s)
Scopus
Bibliographic Citation
Journal of Applied Pharmaceutical Science. Vol 9, No.6 (2019), p.106-110
Suggested Citation
Nuhwa R., Tanasupawat S., Taweechotipatr M., Sitdhipol J., Savarajara A. Bile salt hydrolase activity and cholesterol assimilation of lactic acid bacteria isolated from flowers. Journal of Applied Pharmaceutical Science. Vol 9, No.6 (2019), p.106-110. doi:10.7324/JAPS.2019.90615 Retrieved from: https://hdl.handle.net/20.500.14740/5326
Abstract
A total of 16 isolates of lactic acid bacteria (LAB) from flowers were screened for the bile salt hydrolase activity on MRS (de Man, Rogosa and Sharpe; Difco) agar supplemented with 0.5% (w/v) taurodeoxycholic acid. The isolates were divided into two groups based on their phenotypic characteristics and 16S rRNA gene sequence analysis of the representative isolates. Group I isolates were cocci as the members of genus Enterococcus. Isolates FM1-1, FM1-2, FM12-1, and FM12-2 were identified as Enterococcus durans (100% similarity), isolate FM2-3 was identified as Enterococcus gallinarum (99.92% similarity), while the isolate FM11-2 was identified as Enterococcus lactis (99.77% similarity). Group II isolates were rods as the members of genus Lactobacillus. They were identified as Lactobacillus plantarum subsp. plantarum (the representative isolates, FM3-1 and FM16-2, showed 100% similarity). Eleven isolates, including FM1-1, FM1-2, FM2-3, FM3-1, FM4-2, FM11-2, FM12-1, FM12-2, FM14-1, FM14-2, and FM16-2, exhibited bile salt hydrolase activity. All LAB isolates showed the cholesterol assimilated ability ranged from 9.57% to 51.69%. The isolate FM11-2 efficiently assimilated the cholesterol with 51.69%. © 2019 Rattanatda Nuhwa et al.