Publication:
A sensitive direct ELISA for detection of prostaglandin E2

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dc.contributor.authorAroonrerk N.
dc.contributor.authorSuksamrarn A.
dc.contributor.authorKirtikara K.
dc.date.accessioned2021-04-05T04:32:08Z
dc.date.available2021-04-05T04:32:08Z
dc.date.issued2007
dc.date.issuedBE2550
dc.description.abstractIn order to improve the indirect ELISA for detection of PGE2, a modified direct ELISA technique was developed to measure PGE2 in cell culture supernatants. An evaluation of three types of coating buffer showed that PGE2 was adsorbed efficiently to the solid phase using the gelatin phosphate buffer. The sensitivity of the assay was increased by employing polyclonal rabbit anti-PGE2 antibody dilution of 1/100 and 1% skimmed milk as a blocking solution, with the detection limit of 7.8-500 ng/well. The within-run and between-run coefficients of variation (CV) ranges were 3.2-3.7% and 3.4-3.8%, respectively. A linear standard curve was observed over the range of 0.078-5 μg/mL with a coefficient of determination (r2) of 0.99. Our results indicated that the developed direct ELISA was sensitive and suitable for a quick determination of PGE2 levels from cell culture supernatants. Copyright © Taylor & Francis Group, LLC.
dc.format.mimetypeapplication/pdf
dc.identifier.citationJournal of Immunoassay and Immunochemistry. Vol 28, No.4 (2007), p.319-330
dc.identifier.doi10.1080/15321810701603450
dc.identifier.issn15321819
dc.identifier.other2-s2.0-34548835789
dc.identifier.urihttps://hdl.handle.net/20.500.14740/4335
dc.rights.holderScopus
dc.subject.otherAntibody
dc.subject.otherBuffer
dc.subject.otherGelatin
dc.subject.otherPhosphate buffered saline
dc.subject.otherProstaglandin E2
dc.subject.otherArticle
dc.subject.otherCell culture
dc.subject.otherCorrelation coefficient
dc.subject.otherCulture medium
dc.subject.otherEnzyme linked immunosorbent assay
dc.subject.otherEvaluation
dc.subject.otherHuman
dc.subject.otherHuman cell
dc.subject.otherMilk
dc.subject.otherPolyclonal activation
dc.subject.otherPriority journal
dc.subject.otherSensitivity analysis
dc.subject.otherSolid
dc.subject.otherSupernatant
dc.subject.otherDinoprostone
dc.subject.otherEnzyme-Linked Immunosorbent Assay
dc.subject.otherReagent Kits, Diagnostic
dc.subject.otherReproducibility of Results
dc.subject.otherSensitivity and Specificity
dc.titleA sensitive direct ELISA for detection of prostaglandin E2
dc.typeArticle
dspace.entity.typePublication
swu.datasource.scopushttps://www.scopus.com/inward/record.uri?eid=2-s2.0-34548835789&doi=10.1080%2f15321810701603450&partnerID=40&md5=965a11137310a19c6901482ef61f4c82

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