Publication: A sensitive direct ELISA for detection of prostaglandin E2
2
0
Issued Date
2007
Resource Type
File Type
application/pdf
ISSN
15321819
Other identifier(s)
2-s2.0-34548835789
Rights Holder(s)
Scopus
Bibliographic Citation
Journal of Immunoassay and Immunochemistry. Vol 28, No.4 (2007), p.319-330
Suggested Citation
Aroonrerk N., Suksamrarn A., Kirtikara K. A sensitive direct ELISA for detection of prostaglandin E2. Journal of Immunoassay and Immunochemistry. Vol 28, No.4 (2007), p.319-330. doi:10.1080/15321810701603450 Retrieved from: https://hdl.handle.net/20.500.14740/4335
Author(s)
Abstract
In order to improve the indirect ELISA for detection of PGE2, a modified direct ELISA technique was developed to measure PGE2 in cell culture supernatants. An evaluation of three types of coating buffer showed that PGE2 was adsorbed efficiently to the solid phase using the gelatin phosphate buffer. The sensitivity of the assay was increased by employing polyclonal rabbit anti-PGE2 antibody dilution of 1/100 and 1% skimmed milk as a blocking solution, with the detection limit of 7.8-500 ng/well. The within-run and between-run coefficients of variation (CV) ranges were 3.2-3.7% and 3.4-3.8%, respectively. A linear standard curve was observed over the range of 0.078-5 μg/mL with a coefficient of determination (r2) of 0.99. Our results indicated that the developed direct ELISA was sensitive and suitable for a quick determination of PGE2 levels from cell culture supernatants. Copyright © Taylor & Francis Group, LLC.
Subject(s)
Antibody
Buffer
Gelatin
Phosphate buffered saline
Prostaglandin E2
Article
Cell culture
Correlation coefficient
Culture medium
Enzyme linked immunosorbent assay
Evaluation
Human
Human cell
Milk
Polyclonal activation
Priority journal
Sensitivity analysis
Solid
Supernatant
Dinoprostone
Enzyme-Linked Immunosorbent Assay
Reagent Kits, Diagnostic
Reproducibility of Results
Sensitivity and Specificity
Buffer
Gelatin
Phosphate buffered saline
Prostaglandin E2
Article
Cell culture
Correlation coefficient
Culture medium
Enzyme linked immunosorbent assay
Evaluation
Human
Human cell
Milk
Polyclonal activation
Priority journal
Sensitivity analysis
Solid
Supernatant
Dinoprostone
Enzyme-Linked Immunosorbent Assay
Reagent Kits, Diagnostic
Reproducibility of Results
Sensitivity and Specificity