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Alpha-2-macroglobulin is a modulator of prophenoloxidase system in pacific white shrimp Litopenaeus vannamai

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dc.contributor.authorPonprateep S.
dc.contributor.authorVatanavicharn T.
dc.contributor.authorLo C.F.
dc.contributor.authorTassanakajon A.
dc.contributor.authorRimphanitchayakit V.
dc.date.accessioned2021-04-05T03:22:24Z
dc.date.available2021-04-05T03:22:24Z
dc.date.issued2017
dc.date.issuedBE2560
dc.description.abstractThe shrimp multifunctional protein alpha-2-macroglobulin (A2M) is abundantly expressed in plasma, highly up-regulated upon microbial infection and involved in several immune pathways such as blood clotting system, phagocytosis and melanization. Herein, the function of LvA2M from Litopenaeus vannamei on the prophenoloxidase (proPO) system is reported. The recombinant (r)LvA2M produced strongly and specifically inhibited trypsin and the PO activity in shrimp plasma in a dose-dependent manner. Silencing of LvA2M led to an increase in the PO activity in shrimp plasma although the expression of proPO-associated genes, proPO-activating enzyme (PPAE) and prophenoloxidase (proPO) but not the proPO-activating factor (PPAF) was down-regulated. In Vibrio parahaemolyticus AHPND-infected shrimp, the LvA2M activity was suppressed in an early phase of infection while the PO activity was increased. Thus, the proPO-activating system was regulated by the LvA2M. © 2016 Elsevier Ltd
dc.format.mimetypeapplication/pdf
dc.identifier.citationFish and Shellfish Immunology. Vol 62, (2017), p.68-74
dc.identifier.doi10.1016/j.fsi.2016.12.028
dc.identifier.issn10504648
dc.identifier.other2-s2.0-85009736233
dc.identifier.urihttps://hdl.handle.net/20.500.14740/4227
dc.rights.holderScopus
dc.subject.otherAlpha 2 macroglobulin
dc.subject.otherArthropod protein
dc.subject.otherCatechol oxidase
dc.subject.otherEnzyme precursor
dc.subject.otherPro-phenoloxidase
dc.subject.otherRecombinant protein
dc.subject.otherSerine proteinase
dc.subject.otherAnimal
dc.subject.otherGene expression
dc.subject.otherGene silencing
dc.subject.otherGenetics
dc.subject.otherImmunology
dc.subject.otherInnate immunity
dc.subject.otherMetabolism
dc.subject.otherMicrobiology
dc.subject.otherPenaeidae
dc.subject.otherPhysiology
dc.subject.otherVibrio parahaemolyticus
dc.subject.otherAlpha-Macroglobulins
dc.subject.otherAnimals
dc.subject.otherArthropod Proteins
dc.subject.otherCatechol Oxidase
dc.subject.otherEnzyme Precursors
dc.subject.otherGene Expression
dc.subject.otherGene Knockdown Techniques
dc.subject.otherImmunity, Innate
dc.subject.otherPenaeidae
dc.subject.otherRecombinant Proteins
dc.subject.otherSerine Proteases
dc.subject.otherVibrio parahaemolyticus
dc.titleAlpha-2-macroglobulin is a modulator of prophenoloxidase system in pacific white shrimp Litopenaeus vannamai
dc.typeArticle
dspace.entity.typePublication
swu.datasource.scopushttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85009736233&doi=10.1016%2fj.fsi.2016.12.028&partnerID=40&md5=64e0ff440a83559411269eb998e8cb35

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