Publication:
Alpha-2-macroglobulin is a modulator of prophenoloxidase system in pacific white shrimp Litopenaeus vannamai

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Issued Date

2017

Resource Type

Article

File Type

application/pdf

ISSN

10504648

DOI

10.1016/j.fsi.2016.12.028

Other identifier(s)

2-s2.0-85009736233

Rights Holder(s)

Scopus

Bibliographic Citation

Fish and Shellfish Immunology. Vol 62, (2017), p.68-74

Suggested Citation

Ponprateep S., Vatanavicharn T., Lo C.F., Tassanakajon A., Rimphanitchayakit V. Alpha-2-macroglobulin is a modulator of prophenoloxidase system in pacific white shrimp Litopenaeus vannamai. Fish and Shellfish Immunology. Vol 62, (2017), p.68-74. doi:10.1016/j.fsi.2016.12.028 Retrieved from: https://hdl.handle.net/20.500.14740/4227

Title

Alpha-2-macroglobulin is a modulator of prophenoloxidase system in pacific white shrimp Litopenaeus vannamai

Author(s)

Ponprateep S.
 Vatanavicharn T.
 Lo C.F.
 Tassanakajon A.
 Rimphanitchayakit V.

Abstract

The shrimp multifunctional protein alpha-2-macroglobulin (A2M) is abundantly expressed in plasma, highly up-regulated upon microbial infection and involved in several immune pathways such as blood clotting system, phagocytosis and melanization. Herein, the function of LvA2M from Litopenaeus vannamei on the prophenoloxidase (proPO) system is reported. The recombinant (r)LvA2M produced strongly and specifically inhibited trypsin and the PO activity in shrimp plasma in a dose-dependent manner. Silencing of LvA2M led to an increase in the PO activity in shrimp plasma although the expression of proPO-associated genes, proPO-activating enzyme (PPAE) and prophenoloxidase (proPO) but not the proPO-activating factor (PPAF) was down-regulated. In Vibrio parahaemolyticus AHPND-infected shrimp, the LvA2M activity was suppressed in an early phase of infection while the PO activity was increased. Thus, the proPO-activating system was regulated by the LvA2M. © 2016 Elsevier Ltd

Subject(s)

Alpha 2 macroglobulin
 Arthropod protein
 Catechol oxidase
 Enzyme precursor
 Pro-phenoloxidase
 Recombinant protein
 Serine proteinase
 Animal
 Gene expression
 Gene silencing
 Genetics
 Immunology
 Innate immunity
 Metabolism
 Microbiology
 Penaeidae
 Physiology
 Vibrio parahaemolyticus
 Alpha-Macroglobulins
 Animals
 Arthropod Proteins
 Catechol Oxidase
 Enzyme Precursors
 Gene Expression
 Gene Knockdown Techniques
 Immunity, Innate
 Penaeidae
 Recombinant Proteins
 Serine Proteases
 Vibrio parahaemolyticus

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URI

https://hdl.handle.net/20.500.14740/4227

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Scopus: Year 1983-2021

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