Publication:
Activation of tyrosine hydroxylase RNA expression by water extract of vernonia cinerea in nicotine-pretreated PC12 cells

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dc.contributor.authorSermsuvitayawong K.
dc.contributor.authorWattanachaiyingcharoen R.
dc.contributor.authorKamkaen N.
dc.date.accessioned2021-04-05T03:02:51Z
dc.date.available2021-04-05T03:02:51Z
dc.date.issued2019
dc.date.issuedBE2562
dc.description.abstractBackground: Vernonia cinerea (VC) has been widely applied for smoking cessation. In tobacco users, the activation of dopaminergic neurons via nicotinic cholinergic receptors results in activated brain reward pathways by nicotine. However, molecular mechanism of this Vernonia species in tobacco-addiction is unclear. Objective: The present study aimed to reveal a mechanism whether tyrosine hydroxylase (TH), a rate limiting enzyme in dopaminergic pathway, involved in the bioactivity of Vernonia cinerea on a cellular model of nicotine addict. Materials and Methods: Rat pheochromocytoma 12 (PC12), dopaminergic cell line, was pre-treated and post-treated under medium containing nicotine and medium containing water extract of Vernonia cinerea, respectively. Treated cells were analyzed for the levels of transcripts of TH to determine the functional alteration of genes encoding for this enzyme. Results: TH transcripts was up-regulated under treatment of various concentration of Vernonia extract. Upon cell cytotoxicity, its activation was not resulted from the promotion of cell death. Conclusion: The present study was firstly described the TH activation of bioactive substance in the water extract of Vernonia cinerea. © JOURNAL OF THE MEDICAL ASSOCIATION OF THAILAND| 2019.
dc.format.mimetypeapplication/pdf
dc.identifier.citationJournal of the Medical Association of Thailand. Vol 102, No.7 (2019), p.86-90
dc.identifier.issn1252208
dc.identifier.other2-s2.0-85070536333
dc.identifier.urihttps://hdl.handle.net/20.500.14740/5211
dc.rights.holderมหาวิทยาลัยศรีนครินทรวิโรฒ
dc.subject.otherNicotine
dc.subject.otherPlant extract
dc.subject.otherPlant RNA
dc.subject.otherSmoking cessation agent
dc.subject.otherTyrosine 3 monooxygenase
dc.subject.otherUnclassified drug
dc.subject.otherVernonia cinerea extract
dc.subject.otherAnimal cell
dc.subject.otherArticle
dc.subject.otherCell viability
dc.subject.otherControlled study
dc.subject.otherCyanthillium cinereum
dc.subject.otherCytotoxicity
dc.subject.otherDopaminergic activity
dc.subject.otherDopaminergic nerve cell
dc.subject.otherDrug mechanism
dc.subject.otherGene expression
dc.subject.otherHousekeeping gene
dc.subject.otherMRNA expression level
dc.subject.otherMTT assay
dc.subject.otherMutation
dc.subject.otherNonhuman
dc.subject.otherPlant leaf
dc.subject.otherReal time polymerase chain reaction
dc.subject.otherReverse transcription polymerase chain reaction
dc.subject.otherTobacco dependence
dc.subject.otherUpregulation
dc.titleActivation of tyrosine hydroxylase RNA expression by water extract of vernonia cinerea in nicotine-pretreated PC12 cells
dc.typeArticle
dspace.entity.typePublication
swu.datasource.scopushttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85070536333&partnerID=40&md5=ace13a93ccbcd7d890f8bd6a5872503e

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