Publication: Activation of tyrosine hydroxylase RNA expression by water extract of vernonia cinerea in nicotine-pretreated PC12 cells
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0
Issued Date
2019
Resource Type
File Type
application/pdf
ISSN
1252208
Other identifier(s)
2-s2.0-85070536333
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol 102, No.7 (2019), p.86-90
Suggested Citation
Sermsuvitayawong K., Wattanachaiyingcharoen R., Kamkaen N. Activation of tyrosine hydroxylase RNA expression by water extract of vernonia cinerea in nicotine-pretreated PC12 cells. Journal of the Medical Association of Thailand. Vol 102, No.7 (2019), p.86-90. Retrieved from: https://hdl.handle.net/20.500.14740/5211
Abstract
Background: Vernonia cinerea (VC) has been widely applied for smoking cessation. In tobacco users, the activation of dopaminergic neurons via nicotinic cholinergic receptors results in activated brain reward pathways by nicotine. However, molecular mechanism of this Vernonia species in tobacco-addiction is unclear. Objective: The present study aimed to reveal a mechanism whether tyrosine hydroxylase (TH), a rate limiting enzyme in dopaminergic pathway, involved in the bioactivity of Vernonia cinerea on a cellular model of nicotine addict. Materials and Methods: Rat pheochromocytoma 12 (PC12), dopaminergic cell line, was pre-treated and post-treated under medium containing nicotine and medium containing water extract of Vernonia cinerea, respectively. Treated cells were analyzed for the levels of transcripts of TH to determine the functional alteration of genes encoding for this enzyme. Results: TH transcripts was up-regulated under treatment of various concentration of Vernonia extract. Upon cell cytotoxicity, its activation was not resulted from the promotion of cell death. Conclusion: The present study was firstly described the TH activation of bioactive substance in the water extract of Vernonia cinerea. © JOURNAL OF THE MEDICAL ASSOCIATION OF THAILAND| 2019.
Subject(s)
Nicotine
Plant extract
Plant RNA
Smoking cessation agent
Tyrosine 3 monooxygenase
Unclassified drug
Vernonia cinerea extract
Animal cell
Article
Cell viability
Controlled study
Cyanthillium cinereum
Cytotoxicity
Dopaminergic activity
Dopaminergic nerve cell
Drug mechanism
Gene expression
Housekeeping gene
MRNA expression level
MTT assay
Mutation
Nonhuman
Plant leaf
Real time polymerase chain reaction
Reverse transcription polymerase chain reaction
Tobacco dependence
Upregulation
Plant extract
Plant RNA
Smoking cessation agent
Tyrosine 3 monooxygenase
Unclassified drug
Vernonia cinerea extract
Animal cell
Article
Cell viability
Controlled study
Cyanthillium cinereum
Cytotoxicity
Dopaminergic activity
Dopaminergic nerve cell
Drug mechanism
Gene expression
Housekeeping gene
MRNA expression level
MTT assay
Mutation
Nonhuman
Plant leaf
Real time polymerase chain reaction
Reverse transcription polymerase chain reaction
Tobacco dependence
Upregulation