Publication:
Acute inhibitory effect of alpha-mangostin on sarcoplasmic reticulum calcium-ATPase and myocardial relaxation

2
0

Issued Date

2017

Resource Type

Article

File Type

application/pdf

ISSN

10956670

DOI

10.1002/jbt.21942

Other identifier(s)

2-s2.0-85020256751

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Biochemical and Molecular Toxicology. Vol 31, No.10 (2017)

Suggested Citation

Phungphong S., Kijtawornrat A., De Tombe P.P., Wattanapermpool J., Bupha-Intr T., Suksamrarn S. Acute inhibitory effect of alpha-mangostin on sarcoplasmic reticulum calcium-ATPase and myocardial relaxation. Journal of Biochemical and Molecular Toxicology. Vol 31, No.10 (2017). doi:10.1002/jbt.21942 Retrieved from: https://hdl.handle.net/20.500.14740/4066

Title

Acute inhibitory effect of alpha-mangostin on sarcoplasmic reticulum calcium-ATPase and myocardial relaxation

Author(s)

Phungphong S.
 Kijtawornrat A.
 De Tombe P.P.
 Wattanapermpool J.
 Bupha-Intr T.
 Suksamrarn S.

Abstract

The benefits of α-mangostin for various tissues have been reported, but its effect on the heart has not been clarified. This study aimed to evaluate the effects of α-mangostin on cardiac function. Using a cardiac sarcoplasmic reticulum (SR) membrane preparation, α-mangostin inhibited SR Ca2+-ATPase activity in a dose-dependent manner (IC50 of 6.47 ± 0.7 μM). Its suppressive effect was specific to SR Ca2+-ATPase but not to myofibrillar Ca2+-ATPase. Using isolated cardiomyocytes, 50 μM of α-mangostin significantly increased the duration of cell relengthening and increased the duration of Ca2+ transient decay, suggesting altered myocyte relaxation. The relaxation effect of α-mangostin was also supported in vivo after intravenous infusion. A significant suppression of both peak pressure and rate of ventricular relaxation (–dP/dt) relative to DMSO infusion was observed. The results from the present study demonstrated that α-mangostin exerts specific inhibitory action on SR Ca2+-ATPase activity, leading to myocardial relaxation dysfunction. © 2017 Wiley Periodicals, Inc.

Subject(s)

Adenosine triphosphatase (calcium)
 Adenosine triphosphatase (magnesium)
 Alpha mangostin
 Calcium ion
 Dimethyl sulfoxide
 Sarcoplasmic reticulum calcium transporting adenosine triphosphatase
 Unclassified drug
 Xanthone derivative
 Mangostin
 Sarcoplasmic reticulum calcium transporting adenosine triphosphatase
 Xanthone derivative
 Adult
 Animal cell
 Animal experiment
 Animal tissue
 Article
 Cardiac muscle cell
 Cell isolation
 Concentration response
 Dose response
 Drug dose comparison
 Drug specificity
 Enzyme inhibition
 Heart left ventricle pressure
 Heart left ventricle relaxation
 Heart muscle relaxation
 In vitro study
 In vivo study
 Male
 Nonhuman
 Rat
 Animal
 Antagonists and inhibitors
 Cardiac muscle
 Diastole
 Drug effects
 Heart ventricle
 Leporidae
 Metabolism
 Pathophysiology
 Animals
 Diastole
 Heart Ventricles
 Male
 Myocardium
 Myocytes, Cardiac
 Rabbits
 Sarcoplasmic Reticulum Calcium-Transporting ATPases
 Xanthones

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URI

https://hdl.handle.net/20.500.14740/4066

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Scopus: Year 1983-2021

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