Publication:
In Vitro Study of the Effects of Postbiotic Mouthwash Prototype with Cannabis Extracts on the Reduction of Inflammatory Cytokine: Tumor Necrosis Factor Alpha

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Issued Date

2026-01-01

Resource Type

Article

ISSN

25869981

eISSN

26300559

DOI

10.31584/jhsmr.20251225

Scopus ID

2-s2.0-105024210223

Journal Title

Journal of Health Science and Medical Research

Volume

44

Issue

1

Rights Holder(s)

SCOPUS

Bibliographic Citation

Journal of Health Science and Medical Research Vol.44 No.1 (2026)

Suggested Citation

Nisapar N., Taweechotipatr M., Rungsiyanont S. In Vitro Study of the Effects of Postbiotic Mouthwash Prototype with Cannabis Extracts on the Reduction of Inflammatory Cytokine: Tumor Necrosis Factor Alpha. Journal of Health Science and Medical Research Vol.44 No.1 (2026). doi:10.31584/jhsmr.20251225 Retrieved from: https://hdl.handle.net/20.500.14740/55313

Title

In Vitro Study of the Effects of Postbiotic Mouthwash Prototype with Cannabis Extracts on the Reduction of Inflammatory Cytokine: Tumor Necrosis Factor Alpha

Author(s)

Nisapar N.
 Taweechotipatr M.
 Rungsiyanont S.

Author's Affiliation

Srinakharinwirot University
 Faculty of Medicine, Srinakharinwirot University

Corresponding Author(s)

Nisapar N.

Other Contributor(s)

Srinakharinwirot University

Abstract

Objective: This study evaluated the effects of postbiotic mouthwash containing cannabis extracts (CBD) on the reduction of tumor necrosis factor alpha (TNF-α) levels in vitro. Material and Methods: Seven mouthwash formulations were prepared using CBD at concentrations ranging from 0.25% to 1.0% v/v. TNF-α reduction was assessed using a lipopolysaccharide (LPS) model to activate the THP-1 human monocytic cell line (ATCC, TIB202). The enzyme-linked immunosorbent assay (ELISA) technique was employed to quantify TNF-α. Results: All formulations reduced TNF-α levels compared to the control. The most effective formulations (Preparations 1–4) achieved approximately 91% inhibition. Formulations 5–7 showed inhibition levels of 49.3%, 80.9%, and 82.5%, respectively. Conclusion: The postbiotic mouthwash containing CBD effectively reduced TNF-α levels, indicating its potential for clinical application in inflammation management.

Keyword(s)

Medicine

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URI

https://hdl.handle.net/20.500.14740/55313

Collections

Scopus: Year 2026

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