Publication: Quartz crystal microbalance DNA based biosensor for diagnosis: A review
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0
Issued Date
2012
Resource Type
File Type
application/pdf
ISSN
17265479
Other identifier(s)
2-s2.0-84866644968
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Sensors and Transducers. Vol 143, No.8 (2012), p.44-59
Suggested Citation
Kaewphinit T., Santiwatanakul S., Chansiri K. Quartz crystal microbalance DNA based biosensor for diagnosis: A review. Sensors and Transducers. Vol 143, No.8 (2012), p.44-59. Retrieved from: https://hdl.handle.net/20.500.14740/6956
Author(s)
Abstract
Generally, the detection of particular DNA sequence is carried out by gel electrophoresis of DNA fragments amplified by polymerase chain reaction (PCR) using primers which are specific complementary sequence with the chosen region of DNA. Although gel electrophoresis is simple and effective for detection of PCR products, the use of carcinogenic ethidium bromide as a common stain is risky and hazardous. The DNA quartz crystal microbalance (DNA-QCM) lays the groundwork for incorporating the method for rapid DNA analysis. QCM biosensor is an extremely sensitive mass sensor, capable of measuring picogram levels of mass changes. This is a great potential market for simple, cheap, rapid, and quantitative detection of specific genes. QCM requires any non-labeling such as radio isotope, enzyme, and fluorescence tag. © 2012 IFSA.
Subject(s)
Complementary sequences
DNA analysis
DNA fragment
DNA-based biosensors
Ethidium bromide
Gel electrophoresis
Hybridization
Mass change
PCR products
Picogram level
Potential markets
Quantitative detection
Sensitive mass
Biosensors
Bromine compounds
DNA
Electrophoresis
Isotopes
Quartz crystal microbalances
Radioactive waste vitrification
Polymerase chain reaction
DNA analysis
DNA fragment
DNA-based biosensors
Ethidium bromide
Gel electrophoresis
Hybridization
Mass change
PCR products
Picogram level
Potential markets
Quantitative detection
Sensitive mass
Biosensors
Bromine compounds
DNA
Electrophoresis
Isotopes
Quartz crystal microbalances
Radioactive waste vitrification
Polymerase chain reaction