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Preliminary data on Ascaridia galli infections in Gallus gallus domesticus and the development of a specific primer based on the NADH dehydrogenase subunit 4

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dc.contributor.authorWatcharakranjanaporn T.
dc.contributor.authorSabaijai M.
dc.contributor.authorDunghungzin C.
dc.contributor.authorChontananarth T.
dc.date.accessioned2022-03-10T13:16:43Z
dc.date.available2022-03-10T13:16:43Z
dc.date.issued2021
dc.date.issuedBE2564
dc.description.abstractOne major problem of chicken (Gallus gallus domesticus) farming was various parasitic infections, especially Ascaridia galli that can cause the Ascaridiosis and is commonly found worldwide. The purpose of this study was to investigate the epidemiological situation of gastrointestinal tract parasitic infections and to develop species-specific primer for A. galli detection. A total of 247 chicken gastrointestinal tract specimens from 5 fresh markets in Bangkok. The species-specific primers of A. galli were manually designed using the mitochondrial genome at the NADH dehydrogenase subunit 4 (MT-ND 4) gene. As a result, PCR assays were optimized for the specific PCR product approximately 198 bp with the optimal temperature of 51 °C. In addition, sensitivity tests provided the detection of adult and egg stages at the minimum concentrations of 156.3 ng and 2.8 ng (70 eggs), respectively. This research can be used as preliminary information regarding the epidemic situation of gastrointestinal tract infections in chickens and detection of A. galli infection in definitive hosts, which plans programs for the effective control and prevention of parasitic infections. © 2020, Indian Society for Parasitology.
dc.format.mimetypeapplication/pdf
dc.identifier.citationJournal of Parasitic Diseases. Vol 45, No.2 (2021), p.293-297
dc.identifier.doi10.1007/s12639-020-01321-6
dc.identifier.issn9717196
dc.identifier.other2-s2.0-85096219420
dc.identifier.urihttps://hdl.handle.net/20.500.14740/7789
dc.language.isoeng
dc.rights.holderมหาวิทยาลัยศรีนครินทรวิโรฒ
dc.subject.otherAlcohol
dc.subject.otherReduced nicotinamide adenine dinucleotide dehydrogenase
dc.subject.otherAnimal tissue
dc.subject.otherArticle
dc.subject.otherAscaridia galli
dc.subject.otherAscaridiasis
dc.subject.otherChicken
dc.subject.otherControlled study
dc.subject.otherDNA extraction
dc.subject.otherDNA sequence
dc.subject.otherDrug sensitivity
dc.subject.otherDuodenum
dc.subject.otherEpidemic
dc.subject.otherFeces analysis
dc.subject.otherFlotation
dc.subject.otherGallus gallus
dc.subject.otherGastrointestinal infection
dc.subject.otherGel electrophoresis
dc.subject.otherGene amplification
dc.subject.otherGongylonema pulchrum
dc.subject.otherJejunum
dc.subject.otherMinimum concentration
dc.subject.otherMitochondrial genome
dc.subject.otherMorphology
dc.subject.otherPreliminary data
dc.subject.otherPrevalence
dc.subject.otherReal time polymerase chain reaction
dc.subject.otherRestriction fragment length polymorphism
dc.subject.otherSequence analysis
dc.subject.otherSheep
dc.subject.otherStomach adenocarcinoma
dc.subject.otherTemperature
dc.titlePreliminary data on Ascaridia galli infections in Gallus gallus domesticus and the development of a specific primer based on the NADH dehydrogenase subunit 4
dc.typeArticle
dspace.entity.typePublication
swu.datasource.scopushttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85096219420&doi=10.1007%2fs12639-020-01321-6&partnerID=40&md5=99870dc4d9349087eab0debb884334ec

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