Publication: Preliminary data on Ascaridia galli infections in Gallus gallus domesticus and the development of a specific primer based on the NADH dehydrogenase subunit 4
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Issued Date
2021
Resource Type
Language
eng
File Type
application/pdf
ISSN
9717196
Other identifier(s)
2-s2.0-85096219420
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Journal of Parasitic Diseases. Vol 45, No.2 (2021), p.293-297
Suggested Citation
Watcharakranjanaporn T., Sabaijai M., Dunghungzin C., Chontananarth T. Preliminary data on Ascaridia galli infections in Gallus gallus domesticus and the development of a specific primer based on the NADH dehydrogenase subunit 4. Journal of Parasitic Diseases. Vol 45, No.2 (2021), p.293-297. doi:10.1007/s12639-020-01321-6 Retrieved from: https://hdl.handle.net/20.500.14740/7789
Abstract
One major problem of chicken (Gallus gallus domesticus) farming was various parasitic infections, especially Ascaridia galli that can cause the Ascaridiosis and is commonly found worldwide. The purpose of this study was to investigate the epidemiological situation of gastrointestinal tract parasitic infections and to develop species-specific primer for A. galli detection. A total of 247 chicken gastrointestinal tract specimens from 5 fresh markets in Bangkok. The species-specific primers of A. galli were manually designed using the mitochondrial genome at the NADH dehydrogenase subunit 4 (MT-ND 4) gene. As a result, PCR assays were optimized for the specific PCR product approximately 198 bp with the optimal temperature of 51 °C. In addition, sensitivity tests provided the detection of adult and egg stages at the minimum concentrations of 156.3 ng and 2.8 ng (70 eggs), respectively. This research can be used as preliminary information regarding the epidemic situation of gastrointestinal tract infections in chickens and detection of A. galli infection in definitive hosts, which plans programs for the effective control and prevention of parasitic infections. © 2020, Indian Society for Parasitology.
Subject(s)
Alcohol
Reduced nicotinamide adenine dinucleotide dehydrogenase
Animal tissue
Article
Ascaridia galli
Ascaridiasis
Chicken
Controlled study
DNA extraction
DNA sequence
Drug sensitivity
Duodenum
Epidemic
Feces analysis
Flotation
Gallus gallus
Gastrointestinal infection
Gel electrophoresis
Gene amplification
Gongylonema pulchrum
Jejunum
Minimum concentration
Mitochondrial genome
Morphology
Preliminary data
Prevalence
Real time polymerase chain reaction
Restriction fragment length polymorphism
Sequence analysis
Sheep
Stomach adenocarcinoma
Temperature
Reduced nicotinamide adenine dinucleotide dehydrogenase
Animal tissue
Article
Ascaridia galli
Ascaridiasis
Chicken
Controlled study
DNA extraction
DNA sequence
Drug sensitivity
Duodenum
Epidemic
Feces analysis
Flotation
Gallus gallus
Gastrointestinal infection
Gel electrophoresis
Gene amplification
Gongylonema pulchrum
Jejunum
Minimum concentration
Mitochondrial genome
Morphology
Preliminary data
Prevalence
Real time polymerase chain reaction
Restriction fragment length polymorphism
Sequence analysis
Sheep
Stomach adenocarcinoma
Temperature