Publication:
Barakol suppresses norepinephrine-induced inhibition of spontaneous longitudinal smooth muscle contractions in isolated rat small intestine

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Issued Date

2005

Resource Type

Article

File Type

application/pdf

ISSN

3788741

DOI

10.1016/j.jep.2005.04.017

Other identifier(s)

2-s2.0-24644524706

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Ethnopharmacology. Vol 101, (2005), p.227-232

Suggested Citation

Deachapunya C., Thongsaard W., Poonyachoti S. Barakol suppresses norepinephrine-induced inhibition of spontaneous longitudinal smooth muscle contractions in isolated rat small intestine. Journal of Ethnopharmacology. Vol 101, (2005), p.227-232. doi:10.1016/j.jep.2005.04.017 Retrieved from: https://hdl.handle.net/20.500.14740/6051

Title

Barakol suppresses norepinephrine-induced inhibition of spontaneous longitudinal smooth muscle contractions in isolated rat small intestine

Author(s)

Deachapunya C.
 Thongsaard W.
 Poonyachoti S.

Abstract

The present study aimed to investigate the purgative effects of barakol, the purified extract of Cassia siamea Lam., on the longitudinal smooth muscle contractions of the rat ileum. The extract increased the force of spontaneous muscle contractions in a concentration-dependent manner (EC50 = 0.3 mM). Saxitoxin (0.3 μM) abolished the stimulatory effects of barakol, a result indicating a neural mechanism of action. In addition, atropine (10 μM) but not propanolol (10 μM) or phentolamine (10 μM), partially inhibited barakol-induced smooth muscle contractions suggesting that cholinergic nerves were involved. The motor effects of barakol were further examined in muscle strips treated with catecholamines to suppress spontaneous contractile activity and decrease muscle tone. Norepinephrine or dopamine (10 μM) decreased the amplitude of spontaneous contractions by 72% and 18%, respectively. Pretreatment of the tissues with barakol (1 mM) significantly decreased the inhibitory effect of norepinephrine by 60%, but not that of dopamine. Its ability to potentiate atropine- and saxitoxin-sensitive contractions and inhibit the antimotility actions of norepinephrine suggests that barakol may increase longitudinal smooth muscle contractions by decreasing the inhibitory effect of norepinephrine on excitatory cholinergic motor neurons. Barakol may produce a purgative action in small intestine which may be clinically important in patients with intestinal hypomotility disorders. © 2005 Elsevier Ireland Ltd. All rights reserved.

Subject(s)

Atropine
 Barakol
 Cassia extract
 Catecholamine
 Dopamine
 Noradrenalin
 Phentolamine
 Propranolol
 Saxitoxin
 Unclassified drug
 Animal tissue
 Article
 Cassia siamea
 Cholinergic nerve
 Concentration response
 Drug mechanism
 Ileum contraction
 Intestine innervation
 Male
 Motoneuron
 Nonhuman
 Parasympathetic innervation
 Rat
 Senna
 Smooth muscle contraction
 Smooth muscle tone
 Animals
 Benzopyrans
 Dopamine
 Dose-Response Relationship, Drug
 Ileum
 Male
 Muscle Contraction
 Muscle, Smooth
 Norepinephrine
 Phenalenes
 Rats
 Rats, Wistar
 Senna siamea

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URI

https://hdl.handle.net/20.500.14740/6051

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Scopus: Year 1983-2021

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