Publication: Production of biobutanol from acid-pretreated corncob using Clostridium beijerinckii TISTR 1461: Process optimization studies
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Issued Date
2016
Resource Type
File Type
application/pdf
ISSN
10826068
Other identifier(s)
2-s2.0-84953864756
Rights Holder(s)
Scopus
Bibliographic Citation
Preparative Biochemistry and Biotechnology. Vol 46, No.2 (2016), p.141-149
Suggested Citation
Boonsombuti A., Tangmanasakul K., Nantapipat J., Komolpis K., Luengnaruemitchai A., Wongkasemjit S. Production of biobutanol from acid-pretreated corncob using Clostridium beijerinckii TISTR 1461: Process optimization studies. Preparative Biochemistry and Biotechnology. Vol 46, No.2 (2016), p.141-149. doi:10.1080/10826068.2014.995810 Retrieved from: https://hdl.handle.net/20.500.14740/5636
Abstract
Corncob is a potential feedstock in Thailand that can be used for fermentable sugar production through dilute sulfuric acid pretreatment and enzymatic hydrolysis. To recover high amounts of monomeric sugars from corncob, the sulfuric pretreatment conditions were optimized by using response surface methodology with three independent variables: sulfuric acid concentration, temperature, and time. The highest response of total sugars, 48.84 g/L, was found at 122.78°C, 4.65 min, and 2.82% (v/v) H2SO4. With these conditions, total sugars from the confirmation experiment were 46.29 g/L, with 5.51% error from the predicted value. The hydrolysate was used as a substrate for acetone-butanol-ethanol fermentation to evaluate its potential for microbial growth. The simultaneous saccharification and fermentation (SSF) showed that C. beijerinckii TISTR 1461 can generate acetone-butanol-ethanol products at 11.64 g/L (5.29 g/L acetone, 6.26 g/L butanol, and 0.09 g/L ethanol) instantly using sugars from the hydrolysed corncob with Novozymes 50013 cellulase enzyme without an overliming process. Copyright © Taylor & Francis Group, LLC 2016.
Subject(s)
Acetone
Alcohol
Butanol
Carbohydrate
Cellulase
Sulfuric acid
Chemistry
Clostridium beijerinckii
Fermentation
Growth, development and aging
Hydrolysis
Maize
Metabolism
Microbiology
Particle size
Procedures
Scanning electron microscopy
X ray diffraction
Acetone
Butanols
Carbohydrates
Cellulase
Clostridium beijerinckii
Ethanol
Fermentation
Hydrolysis
Industrial Microbiology
Microscopy, Electron, Scanning
Particle Size
Sulfuric Acids
X-Ray Diffraction
Zea mays
Alcohol
Butanol
Carbohydrate
Cellulase
Sulfuric acid
Chemistry
Clostridium beijerinckii
Fermentation
Growth, development and aging
Hydrolysis
Maize
Metabolism
Microbiology
Particle size
Procedures
Scanning electron microscopy
X ray diffraction
Acetone
Butanols
Carbohydrates
Cellulase
Clostridium beijerinckii
Ethanol
Fermentation
Hydrolysis
Industrial Microbiology
Microscopy, Electron, Scanning
Particle Size
Sulfuric Acids
X-Ray Diffraction
Zea mays