Publication:
Hyaluronic acid graft polymers displaying peptide antigen modulate dendritic cell response in vitro

2
0

Issued Date

2014

Resource Type

Article

File Type

application/pdf

ISSN

15438384

DOI

10.1021/mp4003909

Other identifier(s)

2-s2.0-84891756544

Rights Holder(s)

Scopus

Bibliographic Citation

Molecular Pharmaceutics. Vol 11, No.1 (2014), p.367-373

Suggested Citation

Chittasupho C., Sestak J., Shannon L., Siahaan T.J., Vines C.M., Berkland C. Hyaluronic acid graft polymers displaying peptide antigen modulate dendritic cell response in vitro. Molecular Pharmaceutics. Vol 11, No.1 (2014), p.367-373. doi:10.1021/mp4003909 Retrieved from: https://hdl.handle.net/20.500.14740/7290

Title

Hyaluronic acid graft polymers displaying peptide antigen modulate dendritic cell response in vitro

Author(s)

Chittasupho C.
 Sestak J.
 Shannon L.
 Siahaan T.J.
 Vines C.M.
 Berkland C.

Abstract

A novel oxime grafting scheme was utilized to conjugate an ICAM-1 ligand (LABL), a cellular antigen ovalbumin (OVA), or both peptides simultaneously to hyaluronic acid (HA). Samples of HA only and the various peptide grafted HA were found to bind to dendritic cells (DCs). HA with grafted LABL and OVA showed the greatest binding to DCs. Dendritic cells treated with HA, HA with grafted LABL, or HA with grafted LABL and OVA significantly suppressed T cell and DC conjugate formation and T cell proliferation and reduced proinflammatory cytokine production compared to untreated cells. These results suggest that HA serves as an effective backbone for multivalent ligand presentation for inhibiting T cell response to antigen presentation. In addition, multivalent display of both antigen and an ICAM-1 inhibitor (LABL) may enhance binding to DCs and could potentially disrupt cellular signaling leading to autoimmunity. © 2013 American Chemical Society.

Subject(s)

Gamma interferon
 Hyaluronic acid
 Intercellular adhesion molecule 1
 Interleukin 10
 Interleukin 17
 Interleukin 4
 Ovalbumin
 Polymer
 Tumor necrosis factor alpha
 Animal cell
 Animal experiment
 Antigen presentation
 Article
 Cell adhesion
 Cell culture
 Cell surface
 Conjugation
 Controlled study
 Cytokine production
 Dendritic cell
 Fluorescence microscopy
 Immune response
 In vitro study
 Lymphocyte proliferation
 Macrophage
 Mouse
 Neutrophil
 Nonhuman
 Peptide synthesis
 Priority journal
 Protein binding
 T lymphocyte
 Animals
 Antigen-Presenting Cells
 CD8-Positive T-Lymphocytes
 Cells, Cultured
 Cytokines
 Dendritic Cells
 Enzyme-Linked Immunosorbent Assay
 Hyaluronic Acid
 Intercellular Adhesion Molecule-1
 Lymphocyte Activation
 Mice
 Mice, Inbred C57BL
 Ovalbumin
 Peptide Fragments
 Polymers
 T-Lymphocytes, Regulatory

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URI

https://hdl.handle.net/20.500.14740/7290

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Scopus: Year 1983-2021

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