Publication:
Development of a rapid and sensitive DNA turbidity biosensor test for diagnosis of katG gene in isoniazid resistant Mycobacterium tuberculosis

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dc.contributor.authorCkumdee J.
dc.contributor.authorSantiwatanakul S.
dc.contributor.authorKaewphinit T.
dc.date.accessioned2021-04-05T03:21:54Z
dc.date.available2021-04-05T03:21:54Z
dc.date.issued2017
dc.date.issuedBE2560
dc.description.abstractNovel loop mediated isothermal amplification (LAMP) can also detect one based point mutation drug resistance in Mycobacterium tuberculosis (MTB), by designing inner primers of 5' end specific with the mutant. The reaction was optimized by in-house turbidity biosensor at 61°C, 60 min. It showed the potential to provide a rapid and sensitive method for detecting isoniazid resistant at katG gene position 315 (G→C). The 30 clinical samples were also tested and showed 100% specificity and sensitivity comparing with Genotype® MDR-TB Plus. It could be convenient for detection of MDR-TB in field samples as well. © 2017 IEEE.
dc.format.mimetypeapplication/pdf
dc.identifier.citationProceedings of IEEE Sensors. Vol 2017-December, (2017), p.1-3
dc.identifier.doi10.1109/ICSENS.2017.8234337
dc.identifier.issn19300395
dc.identifier.other2-s2.0-85044288537
dc.identifier.urihttps://hdl.handle.net/20.500.14740/3969
dc.rights.holderScopus
dc.subject.otherBiosensors
dc.subject.otherDrug therapy
dc.subject.otherGenes
dc.subject.otherHydrazine
dc.subject.otherIsotherms
dc.subject.otherPharmacodynamics
dc.subject.otherClinical samples
dc.subject.otherDrug resistance
dc.subject.otherIn-field
dc.subject.otherLoop mediated isothermal amplifications
dc.subject.otherMulti-drug resistant tuberculosis
dc.subject.otherMycobacterium tuberculosis
dc.subject.otherPoint mutations
dc.subject.otherSensitive method
dc.subject.otherTurbidity
dc.titleDevelopment of a rapid and sensitive DNA turbidity biosensor test for diagnosis of katG gene in isoniazid resistant Mycobacterium tuberculosis
dc.typeConference Paper
dspace.entity.typePublication
swu.datasource.scopushttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85044288537&doi=10.1109%2fICSENS.2017.8234337&partnerID=40&md5=d7a8e20b92fc8ba59403e3a5ac39d6f3

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