Publication:
Development of a rapid and sensitive DNA turbidity biosensor test for diagnosis of katG gene in isoniazid resistant Mycobacterium tuberculosis

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Issued Date

2017

Resource Type

Conference Paper

File Type

application/pdf

ISSN

19300395

DOI

10.1109/ICSENS.2017.8234337

Other identifier(s)

2-s2.0-85044288537

Rights Holder(s)

Scopus

Bibliographic Citation

Proceedings of IEEE Sensors. Vol 2017-December, (2017), p.1-3

Suggested Citation

Ckumdee J., Santiwatanakul S., Kaewphinit T. Development of a rapid and sensitive DNA turbidity biosensor test for diagnosis of katG gene in isoniazid resistant Mycobacterium tuberculosis. Proceedings of IEEE Sensors. Vol 2017-December, (2017), p.1-3. doi:10.1109/ICSENS.2017.8234337 Retrieved from: https://hdl.handle.net/20.500.14740/3969

Title

Development of a rapid and sensitive DNA turbidity biosensor test for diagnosis of katG gene in isoniazid resistant Mycobacterium tuberculosis

Author(s)

Ckumdee J.
 Santiwatanakul S.
 Kaewphinit T.

Abstract

Novel loop mediated isothermal amplification (LAMP) can also detect one based point mutation drug resistance in Mycobacterium tuberculosis (MTB), by designing inner primers of 5' end specific with the mutant. The reaction was optimized by in-house turbidity biosensor at 61°C, 60 min. It showed the potential to provide a rapid and sensitive method for detecting isoniazid resistant at katG gene position 315 (G→C). The 30 clinical samples were also tested and showed 100% specificity and sensitivity comparing with Genotype® MDR-TB Plus. It could be convenient for detection of MDR-TB in field samples as well. © 2017 IEEE.

Subject(s)

Biosensors
 Drug therapy
 Genes
 Hydrazine
 Isotherms
 Pharmacodynamics
 Clinical samples
 Drug resistance
 In-field
 Loop mediated isothermal amplifications
 Multi-drug resistant tuberculosis
 Mycobacterium tuberculosis
 Point mutations
 Sensitive method
 Turbidity

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URI

https://hdl.handle.net/20.500.14740/3969

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Scopus: Year 1983-2021

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