Publication:
Poly(DL-lactide)-degrading enzyme production by immobilized Actinomadura keratinilytica strain T16-1 in a 5-L fermenter under various fermentation processes

0
0

Issued Date

2017

Resource Type

Article

File Type

application/pdf

ISSN

7173458

DOI

10.1016/j.ejbt.2017.09.001

Other identifier(s)

2-s2.0-85034060952

Rights Holder(s)

Scopus

Bibliographic Citation

Electronic Journal of Biotechnology. Vol 30, (2017), p.71-76

Suggested Citation

Panyachanakul T., Kitpreechavanich V., Tokuyama S., Krajangsang S. Poly(DL-lactide)-degrading enzyme production by immobilized Actinomadura keratinilytica strain T16-1 in a 5-L fermenter under various fermentation processes. Electronic Journal of Biotechnology. Vol 30, (2017), p.71-76. doi:10.1016/j.ejbt.2017.09.001 Retrieved from: https://hdl.handle.net/20.500.14740/4031

Title

Poly(DL-lactide)-degrading enzyme production by immobilized Actinomadura keratinilytica strain T16-1 in a 5-L fermenter under various fermentation processes

Author(s)

Panyachanakul T.
 Kitpreechavanich V.
 Tokuyama S.
 Krajangsang S.

Abstract

Background Poly(DL-lactic acid), or PDLLA, is a biodegradable polymer that can be hydrolyzed by various types of enzymes. The protease produced by Actinomadura keratinilytica strain T16-1 was previously reported to have PDLLA depolymerase activity. However, few studies have reported on PDLLA-degrading enzyme production by bacteria. Therefore, the aims of this study were to determine a suitable immobilization material for PDLLA-degrading enzyme production and optimize PDLLA-degrading enzyme production by using immobilized A. keratinilytica strain T16-1 under various fermentation process conditions in a stirrer fermenter. Results Among the tested immobilization materials, a scrub pad was the best immobilizer, giving an enzyme activity of 30.03 U/mL in a shake-flask scale. The maximum enzyme activity was obtained at aeration 0.25 vvm, agitation 170 rpm, 45°C, and 48 h of cultivation time. Under these conditions, a PDLLA-degrading enzyme production of 766.33 U/mL with 15.97 U/mL·h productivity was observed using batch fermentation in a 5-L stirrer fermenter. Increased enzyme activity and productivity were observed in repeated-batch (942.67 U/mL and 19.64 U/mL·h) and continuous fermentation (796.43 U/mL and 16.58 U/mL·h) at a dilution rate of 0.013/h. Scaled-up production of the enzyme in a 10-L stirrer bioreactor using the optimized conditions showed a maximum enzyme activity of 578.67 U/mL and a productivity of 12.06 U/mL·h. Conclusions This research successfully scaled-up the enzyme production to 5 and 10 L in a stirrer fermenter and is helpful for many applications of poly(lactic acid). © 2017

Subject(s)

Bacteria
 Biodegradable polymers
 Biodegradation
 Bioreactors
 Cell immobilization
 Enzyme activity
 Enzymes
 Fermentation
 Fermenters
 Lactic acid
 Process control
 Productivity
 Radioactive waste vitrification
 Actinomycete
 Batch fermentation
 Continuous fermentation
 Depolymerase
 Fermentation process
 Optimized conditions
 Plastic wastes
 Poly(DL-lactic acid)
 Enzyme immobilization
 Polylactide
 Proteinase
 Actinomadura
 Actinomadura keratinilytica
 Aeration
 Article
 Biotechnological production
 Continuous fermentation
 Enzyme activity
 Enzyme synthesis
 Fermentation
 Fermentation optimization
 Immobilized cell
 Nonhuman
 Repeated batch fermentation
 Scale up

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/4031

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By