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A specific DNA probe which identifies Babesia bovis in whole blood

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dc.contributor.authorPetchpoo W.
dc.contributor.authorTan-Ariya P.
dc.contributor.authorBoonsaeng V.
dc.contributor.authorBrockelman C.R.
dc.contributor.authorWilairat P.
dc.contributor.authorPanyim S.
dc.date.accessioned2021-04-05T04:33:55Z
dc.date.available2021-04-05T04:33:55Z
dc.date.issued1992
dc.date.issuedBE2535
dc.description.abstractA genomic library of Babesia bovis DNA from the Mexican strain M was constructed in plasmid pUN121 and cloned in Escherichia coli. Several recombinants which hybridized strongly to radioactively labeled B. bovis genomic DNA in an situ screening were selected and further analyzed for those which specifically hybridized to B. bovis DNA. It was found that pMU-B1 had the highest sensitivity, detecting 25 pg of purified B. bovis DNA, and 300 parasites in 10 μl of whole infected blood, or 0.00025% parasiteemia. pMU-B1 contained a 6.0 kb B. bovis DNA insert which did not cross-hybridize to Babesia bigemina, Trypanosoma evansi, Plasmodium falciparum, Anaplasma marginale, Boophilus microplus and cow DNA. In the Southern blot analysis of genomic DNA, pMU-B1 could differentiate between two B. bovis geographic isolates, Mexican strain M and Thai isolate TS4. Thus, the pMU-B1 probe will be useful in the diagnosis of Babesia infection in cattle and ticks, and in the differentiation of B. bovis strains. © 1992.
dc.format.mimetypeapplication/pdf
dc.identifier.citationVeterinary Parasitology. Vol 42, (1992), p.189-198
dc.identifier.doi10.1016/0304-4017(92)90061-D
dc.identifier.issn3044017
dc.identifier.other2-s2.0-0026636008
dc.identifier.urihttps://hdl.handle.net/20.500.14740/7499
dc.rights.holderScopus
dc.subject.otherAnimal experiment
dc.subject.otherArticle
dc.subject.otherBabesiosis
dc.subject.otherCattle
dc.subject.otherControlled study
dc.subject.otherDna probe
dc.subject.otherImmunoblotting
dc.subject.otherNonhuman
dc.subject.otherAnimal
dc.subject.otherBabesia bovis
dc.subject.otherBabesiosis
dc.subject.otherBlotting, Southern
dc.subject.otherCattle
dc.subject.otherCattle Diseases
dc.subject.otherCloning, Molecular
dc.subject.otherDNA Probes
dc.subject.otherDNA, Protozoan
dc.subject.otherErythrocytes
dc.subject.otherGene Library
dc.subject.otherNucleic Acid Hybridization
dc.subject.otherPlasmids
dc.subject.otherRestriction Mapping
dc.subject.otherSensitivity and Specificity
dc.subject.otherSupport, Non-U.S. Gov't
dc.subject.otherTransformation, Genetic
dc.subject.otherAcari
dc.subject.otherAnaplasma marginale
dc.subject.otherAnimalia
dc.subject.otherBabesia
dc.subject.otherBabesia bigemina
dc.subject.otherBabesia bovis
dc.subject.otherBoophilus bovis
dc.subject.otherBoophilus microplus
dc.subject.otherBos taurus
dc.subject.otherEscherichia coli
dc.subject.otherPlasmodium falciparum
dc.subject.otherTrypanosoma
dc.subject.otherTrypanosoma evansi
dc.titleA specific DNA probe which identifies Babesia bovis in whole blood
dc.typeArticle
dspace.entity.typePublication
swu.datasource.scopushttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0026636008&doi=10.1016%2f0304-4017%2892%2990061-D&partnerID=40&md5=3c381434c2b679e8f500ec8c6e45b827

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