Publication:
A specific DNA probe which identifies Babesia bovis in whole blood

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Issued Date

1992

Resource Type

Article

File Type

application/pdf

ISSN

3044017

DOI

10.1016/0304-4017(92)90061-D

Other identifier(s)

2-s2.0-0026636008

Rights Holder(s)

Scopus

Bibliographic Citation

Veterinary Parasitology. Vol 42, (1992), p.189-198

Suggested Citation

Petchpoo W., Tan-Ariya P., Boonsaeng V., Brockelman C.R., Wilairat P., Panyim S. A specific DNA probe which identifies Babesia bovis in whole blood. Veterinary Parasitology. Vol 42, (1992), p.189-198. doi:10.1016/0304-4017(92)90061-D Retrieved from: https://hdl.handle.net/20.500.14740/7499

Title

A specific DNA probe which identifies Babesia bovis in whole blood

Author(s)

Petchpoo W.
 Tan-Ariya P.
 Boonsaeng V.
 Brockelman C.R.
 Wilairat P.
 Panyim S.

Abstract

A genomic library of Babesia bovis DNA from the Mexican strain M was constructed in plasmid pUN121 and cloned in Escherichia coli. Several recombinants which hybridized strongly to radioactively labeled B. bovis genomic DNA in an situ screening were selected and further analyzed for those which specifically hybridized to B. bovis DNA. It was found that pMU-B1 had the highest sensitivity, detecting 25 pg of purified B. bovis DNA, and 300 parasites in 10 μl of whole infected blood, or 0.00025% parasiteemia. pMU-B1 contained a 6.0 kb B. bovis DNA insert which did not cross-hybridize to Babesia bigemina, Trypanosoma evansi, Plasmodium falciparum, Anaplasma marginale, Boophilus microplus and cow DNA. In the Southern blot analysis of genomic DNA, pMU-B1 could differentiate between two B. bovis geographic isolates, Mexican strain M and Thai isolate TS4. Thus, the pMU-B1 probe will be useful in the diagnosis of Babesia infection in cattle and ticks, and in the differentiation of B. bovis strains. © 1992.

Subject(s)

Animal experiment
 Article
 Babesiosis
 Cattle
 Controlled study
 Dna probe
 Immunoblotting
 Nonhuman
 Animal
 Babesia bovis
 Babesiosis
 Blotting, Southern
 Cattle
 Cattle Diseases
 Cloning, Molecular
 DNA Probes
 DNA, Protozoan
 Erythrocytes
 Gene Library
 Nucleic Acid Hybridization
 Plasmids
 Restriction Mapping
 Sensitivity and Specificity
 Support, Non-U.S. Gov't
 Transformation, Genetic
 Acari
 Anaplasma marginale
 Animalia
 Babesia
 Babesia bigemina
 Babesia bovis
 Boophilus bovis
 Boophilus microplus
 Bos taurus
 Escherichia coli
 Plasmodium falciparum
 Trypanosoma
 Trypanosoma evansi

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URI

https://hdl.handle.net/20.500.14740/7499

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Scopus: Year 1983-2021

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