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Development and evaluation of a loop-mediated isothermal amplification combined with au-nanoprobe assay for rapid detection of Mycobacterium tuberculosis

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dc.contributor.authorKaewphinit T.
dc.contributor.authorCkumdee J.
dc.contributor.authorChansiri K.
dc.contributor.authorSantiwatanakul S.
dc.date.accessioned2021-04-05T03:22:19Z
dc.date.available2021-04-05T03:22:19Z
dc.date.issued2017
dc.date.issuedBE2560
dc.description.abstractLoop-mediated isothermal amplification (LAMP) has been proposed as an inexpensive and easy to perform assay for molecular diagnostics. We present a novel strategy for the detection of LAMP amplicons derived from Mycobacterium tuberculosis by the use of Au-nanoprobes. When applied to a total of 93 clinical specimens, the LAMP assay demonstrated sensitivity and specificity higher than that of polymerase chain reaction and culture. The Au-nanoprobe augmented LAMP test platform with its advantages of robust reagents and a simple colorimetric detection method can be adapted easily for the rapid detection of other infectious disease agents at a low cost. © 2017 Indian Journal of Medical Microbiology Published by Wolters Kluwer -Medknow.
dc.format.mimetypeapplication/pdf
dc.identifier.citationIndian Journal of Medical Microbiology. Vol 35, No.2 (2017), p.302-304
dc.identifier.doi10.4103/ijmm.IJMM_15_333
dc.identifier.issn2550857
dc.identifier.other2-s2.0-85022222151
dc.identifier.urihttps://hdl.handle.net/20.500.14740/4189
dc.rights.holderScopus
dc.subject.otherArticle
dc.subject.otherAu nanoprobe assay
dc.subject.otherBacterium detection
dc.subject.otherColorimetry
dc.subject.otherControlled study
dc.subject.otherDiagnostic procedure
dc.subject.otherLoop mediated isothermal amplification
dc.subject.otherMicroscopy
dc.subject.otherMycobacterium tuberculosis
dc.subject.otherNonhuman
dc.subject.otherPolymerase chain reaction
dc.subject.otherPredictive value
dc.subject.otherSensitivity and specificity
dc.subject.otherSputum culture
dc.subject.otherSputum smear
dc.subject.otherTuberculosis
dc.subject.otherTuberculosis rapid test
dc.subject.otherCost
dc.subject.otherEvaluation study
dc.subject.otherGenetics
dc.subject.otherHuman
dc.subject.otherIsolation and purification
dc.subject.otherMolecular diagnosis
dc.subject.otherMycobacterium tuberculosis
dc.subject.otherNanotechnology
dc.subject.otherNucleic acid amplification
dc.subject.otherProcedures
dc.subject.otherTime factor
dc.subject.otherTuberculosis
dc.subject.otherGold
dc.subject.otherMetal nanoparticle
dc.subject.otherColorimetry
dc.subject.otherCosts and Cost Analysis
dc.subject.otherGold
dc.subject.otherHumans
dc.subject.otherMetal Nanoparticles
dc.subject.otherMolecular Diagnostic Techniques
dc.subject.otherMycobacterium tuberculosis
dc.subject.otherNanotechnology
dc.subject.otherNucleic Acid Amplification Techniques
dc.subject.otherSensitivity and Specificity
dc.subject.otherTime Factors
dc.subject.otherTuberculosis
dc.titleDevelopment and evaluation of a loop-mediated isothermal amplification combined with au-nanoprobe assay for rapid detection of Mycobacterium tuberculosis
dc.typeArticle
dspace.entity.typePublication
swu.datasource.scopushttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85022222151&doi=10.4103%2fijmm.IJMM_15_333&partnerID=40&md5=f3db7e86384828e5b06f548d5e592dce

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