Publication:
Development and evaluation of a loop-mediated isothermal amplification combined with au-nanoprobe assay for rapid detection of Mycobacterium tuberculosis

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Issued Date

2017

Resource Type

Article

File Type

application/pdf

ISSN

2550857

DOI

10.4103/ijmm.IJMM_15_333

Other identifier(s)

2-s2.0-85022222151

Rights Holder(s)

Scopus

Bibliographic Citation

Indian Journal of Medical Microbiology. Vol 35, No.2 (2017), p.302-304

Suggested Citation

Kaewphinit T., Ckumdee J., Chansiri K., Santiwatanakul S. Development and evaluation of a loop-mediated isothermal amplification combined with au-nanoprobe assay for rapid detection of Mycobacterium tuberculosis. Indian Journal of Medical Microbiology. Vol 35, No.2 (2017), p.302-304. doi:10.4103/ijmm.IJMM_15_333 Retrieved from: https://hdl.handle.net/20.500.14740/4189

Title

Development and evaluation of a loop-mediated isothermal amplification combined with au-nanoprobe assay for rapid detection of Mycobacterium tuberculosis

Author(s)

Kaewphinit T.
 Ckumdee J.
 Chansiri K.
 Santiwatanakul S.

Abstract

Loop-mediated isothermal amplification (LAMP) has been proposed as an inexpensive and easy to perform assay for molecular diagnostics. We present a novel strategy for the detection of LAMP amplicons derived from Mycobacterium tuberculosis by the use of Au-nanoprobes. When applied to a total of 93 clinical specimens, the LAMP assay demonstrated sensitivity and specificity higher than that of polymerase chain reaction and culture. The Au-nanoprobe augmented LAMP test platform with its advantages of robust reagents and a simple colorimetric detection method can be adapted easily for the rapid detection of other infectious disease agents at a low cost. © 2017 Indian Journal of Medical Microbiology Published by Wolters Kluwer -Medknow.

Subject(s)

Article
 Au nanoprobe assay
 Bacterium detection
 Colorimetry
 Controlled study
 Diagnostic procedure
 Loop mediated isothermal amplification
 Microscopy
 Mycobacterium tuberculosis
 Nonhuman
 Polymerase chain reaction
 Predictive value
 Sensitivity and specificity
 Sputum culture
 Sputum smear
 Tuberculosis
 Tuberculosis rapid test
 Cost
 Evaluation study
 Genetics
 Human
 Isolation and purification
 Molecular diagnosis
 Mycobacterium tuberculosis
 Nanotechnology
 Nucleic acid amplification
 Procedures
 Time factor
 Tuberculosis
 Gold
 Metal nanoparticle
 Colorimetry
 Costs and Cost Analysis
 Gold
 Humans
 Metal Nanoparticles
 Molecular Diagnostic Techniques
 Mycobacterium tuberculosis
 Nanotechnology
 Nucleic Acid Amplification Techniques
 Sensitivity and Specificity
 Time Factors
 Tuberculosis

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URI

https://hdl.handle.net/20.500.14740/4189

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Scopus: Year 1983-2021

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