Publication: Simultaneous injection effective mixing flow analysis of urinary albumin using dye-binding reaction
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Issued Date
2012
Resource Type
File Type
application/pdf
ISSN
399140
Other identifier(s)
2-s2.0-84864281015
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Talanta. Vol 96, No. (2012), p.50-54
Suggested Citation
Ratanawimarnwong N., Ponhong K., Teshima N., Nacapricha D., Grudpan K., Sakai T., Motomizu S. Simultaneous injection effective mixing flow analysis of urinary albumin using dye-binding reaction. Talanta. Vol 96, No. (2012), p.50-54. doi:10.1016/j.talanta.2012.02.027 Retrieved from: https://hdl.handle.net/20.500.14740/7008
Abstract
A new four-channel simultaneous injection effective mixing flow analysis (SIEMA) system has been assembled for the determination of urinary albumin. The SIEMA system consisted of a syringe pump, two 5-way cross connectors, four holding coils, five 3-way solenoid valves, a 50-cm long mixing coil and a spectrophotometer. Tetrabromophenol blue anion (TBPB) in Triton X-100 micelle reacted with albumin at pH 3.2 to form a blue ion complex with a λmax 625 nm. TBPB, Triton X-100, acetate buffer and albumin standard solutions were aspirated into four individual holding coils by a syringe pump and then the aspirated zones were simultaneously pushed in the reverse direction to the detector flow cell. Baseline drift, due to adsorption of TBPB-albumin complex on the wall of the hydrophobic PTFE tubing, was minimized by aspiration of Triton X-100 and acetate buffer solutions between samples. The calibration graph was linear in the range of 10-50 μg/mL and the detection limit for albumin (3σ) was 0.53 μg/mL. The RSD (n = 11) at 30 μg/mL was 1.35%. The sample throughput was 37/h. With a 10-fold dilution, interference from urine matrix was removed. The proposed method has advantages in terms of simple automation operation and short analysis time. © 2012 Elsevier B.V. All rights reserved.