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Expression and characterization of Cu/Zn superoxide dismutase from Wuchereria bancrofti

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dc.contributor.authorKhawsak P.
dc.contributor.authorKanjanavas P.
dc.contributor.authorKiatsomchai P.
dc.contributor.authorChansiri K.
dc.date.accessioned2021-04-05T03:34:29Z
dc.date.available2021-04-05T03:34:29Z
dc.date.issued2012
dc.date.issuedBE2555
dc.description.abstractThe Cu/Zn superoxide dismutase gene from Wuchereria bancrofti (Cu/Zn WbSOD) was isolated by PCR using degeneracy primers. The complete Cu/Zn WbSOD consisted of 1,032 nucleotides containing 4 exons (477 nucleotides) and 3 introns. The molecular phylogenetic analysis of the Cu/Zn WbSOD gene in comparison with those from other organisms revealed that the gene was classified in the same clade to those of filarial Brugia malayi and Brugia pahangi (bootstrap value at 90). The nucleotide and deduced amino acid sequences of Cu/Zn WbSOD exhibited the similarity to those of intracellular Cu/Zn SOD of B. malayi and B. pahangi. The amino acid comparison of Cu/Zn WbSOD to others revealed that the binding sites and active sites were conserved. The expression of this gene yielded 16.366 kDa in size. After Ni-IDA column purification, the enzyme showed specific activity of 8.5 U/mg and 42.1% yield. The enzyme activity was inhibited when 6 mM KCN was added. © Springer-Verlag 2011.
dc.format.mimetypeapplication/pdf
dc.identifier.citationParasitology Research. Vol 110, No.2 (2012), p.629-636
dc.identifier.doi10.1007/s00436-011-2532-z
dc.identifier.issn9320113
dc.identifier.other2-s2.0-84857059728
dc.identifier.urihttps://hdl.handle.net/20.500.14740/7110
dc.rights.holderScopus
dc.subject.otherCopper zinc superoxide dismutase
dc.subject.otherNucleotide
dc.subject.otherPotassium cyanide
dc.subject.otherAmino acid sequence
dc.subject.otherArticle
dc.subject.otherBinding site
dc.subject.otherBootstrapping
dc.subject.otherBrugia malayi
dc.subject.otherBrugia pahangi
dc.subject.otherCladistics
dc.subject.otherControlled study
dc.subject.otherEnzyme active site
dc.subject.otherEnzyme activity
dc.subject.otherEnzyme analysis
dc.subject.otherEnzyme isolation
dc.subject.otherExon
dc.subject.otherFilaria
dc.subject.otherGene
dc.subject.otherGene expression
dc.subject.otherHuman
dc.subject.otherHuman tissue
dc.subject.otherIntron
dc.subject.otherMolecular phylogeny
dc.subject.otherNonhuman
dc.subject.otherNucleotide sequence
dc.subject.otherPolymerase chain reaction
dc.subject.otherPriority journal
dc.subject.otherProtein expression
dc.subject.otherWuchereria bancrofti
dc.subject.otherAnimals
dc.subject.otherBinding Sites
dc.subject.otherCatalytic Domain
dc.subject.otherCloning, Molecular
dc.subject.otherCluster Analysis
dc.subject.otherConserved Sequence
dc.subject.otherDNA Primers
dc.subject.otherDNA, Helminth
dc.subject.otherEnzyme Inhibitors
dc.subject.otherExons
dc.subject.otherGene Expression
dc.subject.otherIntrons
dc.subject.otherMolecular Sequence Data
dc.subject.otherMolecular Weight
dc.subject.otherPhylogeny
dc.subject.otherPolymerase Chain Reaction
dc.subject.otherPotassium Cyanide
dc.subject.otherRecombinant Proteins
dc.subject.otherSequence Analysis, DNA
dc.subject.otherSequence Homology, Amino Acid
dc.subject.otherSuperoxide Dismutase
dc.subject.otherWuchereria bancrofti
dc.subject.otherBrugia malayi
dc.subject.otherBrugia pahangi
dc.subject.otherWuchereria bancrofti
dc.titleExpression and characterization of Cu/Zn superoxide dismutase from Wuchereria bancrofti
dc.typeArticle
dspace.entity.typePublication
swu.datasource.scopushttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84857059728&doi=10.1007%2fs00436-011-2532-z&partnerID=40&md5=221f7a69f0ec7c272a5ce7d65ed12488

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