Publication:
Expression and characterization of Cu/Zn superoxide dismutase from Wuchereria bancrofti

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Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

9320113

DOI

10.1007/s00436-011-2532-z

Other identifier(s)

2-s2.0-84857059728

Rights Holder(s)

Scopus

Bibliographic Citation

Parasitology Research. Vol 110, No.2 (2012), p.629-636

Suggested Citation

Khawsak P., Kanjanavas P., Kiatsomchai P., Chansiri K. Expression and characterization of Cu/Zn superoxide dismutase from Wuchereria bancrofti. Parasitology Research. Vol 110, No.2 (2012), p.629-636. doi:10.1007/s00436-011-2532-z Retrieved from: https://hdl.handle.net/20.500.14740/7110

Title

Expression and characterization of Cu/Zn superoxide dismutase from Wuchereria bancrofti

Author(s)

Khawsak P.
 Kanjanavas P.
 Kiatsomchai P.
 Chansiri K.

Abstract

The Cu/Zn superoxide dismutase gene from Wuchereria bancrofti (Cu/Zn WbSOD) was isolated by PCR using degeneracy primers. The complete Cu/Zn WbSOD consisted of 1,032 nucleotides containing 4 exons (477 nucleotides) and 3 introns. The molecular phylogenetic analysis of the Cu/Zn WbSOD gene in comparison with those from other organisms revealed that the gene was classified in the same clade to those of filarial Brugia malayi and Brugia pahangi (bootstrap value at 90). The nucleotide and deduced amino acid sequences of Cu/Zn WbSOD exhibited the similarity to those of intracellular Cu/Zn SOD of B. malayi and B. pahangi. The amino acid comparison of Cu/Zn WbSOD to others revealed that the binding sites and active sites were conserved. The expression of this gene yielded 16.366 kDa in size. After Ni-IDA column purification, the enzyme showed specific activity of 8.5 U/mg and 42.1% yield. The enzyme activity was inhibited when 6 mM KCN was added. © Springer-Verlag 2011.

Subject(s)

Copper zinc superoxide dismutase
 Nucleotide
 Potassium cyanide
 Amino acid sequence
 Article
 Binding site
 Bootstrapping
 Brugia malayi
 Brugia pahangi
 Cladistics
 Controlled study
 Enzyme active site
 Enzyme activity
 Enzyme analysis
 Enzyme isolation
 Exon
 Filaria
 Gene
 Gene expression
 Human
 Human tissue
 Intron
 Molecular phylogeny
 Nonhuman
 Nucleotide sequence
 Polymerase chain reaction
 Priority journal
 Protein expression
 Wuchereria bancrofti
 Animals
 Binding Sites
 Catalytic Domain
 Cloning, Molecular
 Cluster Analysis
 Conserved Sequence
 DNA Primers
 DNA, Helminth
 Enzyme Inhibitors
 Exons
 Gene Expression
 Introns
 Molecular Sequence Data
 Molecular Weight
 Phylogeny
 Polymerase Chain Reaction
 Potassium Cyanide
 Recombinant Proteins
 Sequence Analysis, DNA
 Sequence Homology, Amino Acid
 Superoxide Dismutase
 Wuchereria bancrofti
 Brugia malayi
 Brugia pahangi
 Wuchereria bancrofti

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URI

https://hdl.handle.net/20.500.14740/7110

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Scopus: Year 1983-2021

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