Publication:
Graphene-polyaniline modified electrochemical droplet-based microfluidic sensor for high-throughput determination of 4-aminophenol

2
0

Issued Date

2016

Resource Type

Article

File Type

application/pdf

ISSN

32670

DOI

10.1016/j.aca.2016.03.010

Other identifier(s)

2-s2.0-84966473932

Rights Holder(s)

Scopus

Bibliographic Citation

Analytica Chimica Acta. Vol 925, (2016), p.51-60

Suggested Citation

Rattanarat P., Suea-Ngam A., Ruecha N., Siangproh W., Henry C.S., Srisa-Art M., Chailapakul O. Graphene-polyaniline modified electrochemical droplet-based microfluidic sensor for high-throughput determination of 4-aminophenol. Analytica Chimica Acta. Vol 925, (2016), p.51-60. doi:10.1016/j.aca.2016.03.010 Retrieved from: https://hdl.handle.net/20.500.14740/5344

Title

Graphene-polyaniline modified electrochemical droplet-based microfluidic sensor for high-throughput determination of 4-aminophenol

Author(s)

Rattanarat P.
 Suea-Ngam A.
 Ruecha N.
 Siangproh W.
 Henry C.S.
 Srisa-Art M.
 Chailapakul O.

Abstract

We report herein the first development of graphene-polyaniline modified carbon paste electrode (G-PANI/CPE) coupled with droplet-based microfluidic sensor for high-throughput detection of 4-aminophenol (4-AP) in pharmaceutical paracetamol (PA) formulations. A simple T-junction microfluidic platform using an oil flow rate of 1.8 μL/min and an aqueous flow rate of 0.8 μL/min was used to produce aqueous testing microdroplets continuously. The microchannel was designed to extend the aqueous droplet to cover all 3 electrodes, allowing for electrochemical measurements in a single droplet. Parameters including flow rate, water fraction, and applied detection potential (Edet) were investigated to obtain optimal conditions. Using G-PANI/CPE significantly increased the current response for both cyclic voltammetric detections of ferri/ferrocyanide [Fe(CN)6]3-/4- (10 times) and 4-AP (2 times), compared to an unmodified electrode. Using the optimized conditions in the droplet system, 4-AP in the presence of PA was selectively determined. The linear range of 4-AP was 50-500 μM (R2 = 0.99), limit of detection (LOD, S/N = 3) was 15.68 μM, and limit of quantification (LOQ, S/N = 10) was 52.28 μM. Finally, the system was used to determine 4-AP spiked in commercial PA liquid samples and the amounts of 4-AP were found in good agreement with those obtained from the conventional capillary zone electrophoresis/UV-Visible spectrophotometry (CZE/UV-Vis). The proposed microfluidic device could be employed for a high-throughput screening (at least 60 samples h-1) of pharmaceutical purity requiring low sample and reagent consumption. © 2016 Elsevier B.V.

Subject(s)

Capillary electrophoresis
 Carbon
 Chemical detection
 Drops
 Drug products
 Electrochemical electrodes
 Electrodes
 Electrophoresis
 Graphene
 Intersections
 Polyaniline
 Screening
 Throughput
 4-aminophenols
 Capillary zone electrophoresis
 Droplet-based microfluidics
 ELectrochemical detection
 Electrochemical measurements
 High throughput screening
 Modified carbon paste electrode
 Paracetamol
 Microfluidics
 Aminophenol
 Graphene
 Polyaniline
 4-aminophenol
 Aminophenol derivative
 Aniline derivative
 Graphite
 Polyaniline
 Adsorption
 Article
 Controlled study
 Cyclic potentiometry
 Dispersion
 Electrochemical detection
 Flow rate
 High throughput screening
 Hydrodynamics
 Limit of detection
 Limit of quantitation
 Microfluidics
 Oxidation reduction potential
 Priority journal
 Sensitivity and specificity
 Signal noise ratio
 Chemistry
 Devices
 Electrochemical analysis
 Microfluidics
 Procedures
 Scanning electron microscopy
 Transmission electron microscopy
 Aminophenols
 Aniline Compounds
 Electrochemical Techniques
 Graphite
 High-Throughput Screening Assays
 Microfluidics
 Microscopy, Electron, Scanning
 Microscopy, Electron, Transmission

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URI

https://hdl.handle.net/20.500.14740/5344

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Scopus: Year 1983-2021

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