Publication: Streptomyces endocoffeicus sp. nov., an endophytic actinomycete isolated from Coffea arabica (L.)
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Issued Date
2021
Resource Type
Language
eng
File Type
application/pdf
ISSN
36072
Other identifier(s)
2-s2.0-85114336090
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology. Vol 114, No.11 (2021), p.1889-1898
Suggested Citation
Nammali A., Intaraudom C., Pittayakhajonwut P., Suriyachadkun C., Tadtong S., Tanasupawat S., Thawai C. Streptomyces endocoffeicus sp. nov., an endophytic actinomycete isolated from Coffea arabica (L.). Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology. Vol 114, No.11 (2021), p.1889-1898. doi:10.1007/s10482-021-01648-x Retrieved from: https://hdl.handle.net/20.500.14740/8147
Abstract
An aerobic, non-motile, Gram-stain positive actinomycete, designated strain CA3R110T, was isolated from the surface-sterilised root of Coffea arabica L. collected from Lampang Province, Thailand. 16S rRNA gene sequence analysis indicated that strain CA3R110T was a member of the genus Streptomyces and showed the closest similarities to Streptomyces buecherae AC541T (99.2%), followed by Streptomyces rapamycinicus NRRL B-5491T (99.1%), Streptomyces luteoverticillatus NBRC 3840T (99.1%), Streptomyces coerulescens NBRC 12758T (99.1%), and Streptomyces iranensis HM 35T (99.0%). Strain CA3R110T contained LL-diaminopimelic acid in cell peptidoglycan, MK-9(H6), and MK-9(H8) as major menaquinone, iso-C16:0,iso-C15:0, C16:0 as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside were detected in the cell. The chemotaxonomic characteristics possessed the typical properties of the genus Streptomyces. A low digital DNA–DNA hybridization (< 55.7%) and average nucleotide identity-blast (ANIb) (< 92.2%) values revealed that strain CA3R110T could be distinguished from any known Streptomyces species. With the differences in phenotypic and genotypic data, strain CA3R110T represents a novel species of genus Streptomyces, for which the name Streptomyces endocoffeicus sp. nov. is proposed. The type strain is CA3R110T (= TBRC 11245T = NBRC 114296T). © 2021, The Author(s), under exclusive licence to Springer Nature Switzerland AG.
Subject(s)
Bacterial DNA
Phospholipid
RNA 16S
Actinobacteria
Bacterium identification
Coffea
DNA base composition
DNA sequence
Genetics
Phylogeny
Streptomyces
Thailand
Actinobacteria
Bacterial Typing Techniques
Base Composition
Coffea
DNA, Bacterial
Phospholipids
Phylogeny
RNA, Ribosomal, 16S
Sequence Analysis, DNA
Streptomyces
Thailand
Phospholipid
RNA 16S
Actinobacteria
Bacterium identification
Coffea
DNA base composition
DNA sequence
Genetics
Phylogeny
Streptomyces
Thailand
Actinobacteria
Bacterial Typing Techniques
Base Composition
Coffea
DNA, Bacterial
Phospholipids
Phylogeny
RNA, Ribosomal, 16S
Sequence Analysis, DNA
Streptomyces
Thailand