Publication:
Simple in-house flow-injection capillary electrophoresis with capacitively coupled contactless conductivity method for the determination of colistin

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Issued Date

2015

Resource Type

Article

File Type

application/pdf

ISSN

16159306

DOI

10.1002/jssc.201401101

Other identifier(s)

2-s2.0-84925349843

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Separation Science. Vol 38, No.6 (2015), p.1035-1041

Suggested Citation

Chaisuwan P., Moonta T., Sangcakul A., Nacapricha D., Wilairat P., Uraisin K. Simple in-house flow-injection capillary electrophoresis with capacitively coupled contactless conductivity method for the determination of colistin. Journal of Separation Science. Vol 38, No.6 (2015), p.1035-1041. doi:10.1002/jssc.201401101 Retrieved from: https://hdl.handle.net/20.500.14740/6168

Title

Simple in-house flow-injection capillary electrophoresis with capacitively coupled contactless conductivity method for the determination of colistin

Author(s)

Chaisuwan P.
 Moonta T.
 Sangcakul A.
 Nacapricha D.
 Wilairat P.
 Uraisin K.

Abstract

An in-house flow-injection capillary electrophoresis with capacitively coupled contactless conductivity detection method was developed for the direct measurement of colistin in pharmaceutical samples. The flow injection and capillary electrophoresis systems are connected by an acrylic interface. Capillary electrophoresis separation is achieved within 2 min using a background electrolyte solution of 5 mM 2-morpholinoethanesulfonic acid and 5 mM histidine (pH 6). The flow-injection section allows for convenient filling of the capillary and sample introduction without the use of a pressure/vacuum manifold. Capacitively coupled contactless conductivity detection is employed since colistin has no chromophore but is cationic at pH 6. Calibration curve is linear from 20 to 150 mg/L, with a correlation coefficient (r2) of 0.997. The limit of quantitation is 20 mg/L. The developed method provides precision, simplicity, and short analysis time. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Subject(s)

Amino acids
 Chromophores
 Back ground electrolyte
 Capacitively coupled contactless conductivity detection
 Capillary electrophoresis separation
 Colistin
 Correlation coefficient
 Flow injection
 Limit of quantitations
 Polymyxin E
 Capillary electrophoresis
 2 morpholinoethanesulfonic acid
 Amino acid
 Colistin
 Histidine
 Unclassified drug
 Antiinfective agent
 Colistin
 Article
 Capillary electrophoresis
 Chromatophore
 Conductance
 Drug determination
 Flow injection capillary electrophoresis
 Hydrodynamics
 Limit of quantitation
 Membrane filter
 PH
 Priority journal
 Capillary electrophoresis
 Devices
 Evaluation study
 Flow injection analysis
 Procedures
 Anti-Bacterial Agents
 Colistin
 Electrophoresis, Capillary
 Flow Injection Analysis

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URI

https://hdl.handle.net/20.500.14740/6168

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Scopus: Year 1983-2021

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